MKK6 (D31D1) Rabbit mAb

• 产品编号：CST-8550S      品牌：CST       原厂货号：8550S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

MKK6 (D31D1) Rabbit mAb recognizes endogenous levels of total MKK6 protein. This antibody does not cross-react with MKK3 or other members of the MKK family.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant, full-length MKK6 expressed in E. coli.

原厂资料：

Specificity / Sensitivity

MKK6 (D31D1) Rabbit mAb recognizes endogenous levels of total MKK6 protein. This antibody does not cross-react with MKK3 or other members of the MKK family.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant, full-length MKK6 expressed in E. coli.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr193 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.