Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb

• 产品编号：CST-8548S      品牌：CST       原厂货号：8548S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

只有当丝氨酸（1756位）磷酸化时，Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb兔单抗检测内源性的IP3受体。该单克隆抗体通过用合成肽免疫动物制备，该合成肽与人IP3 受体蛋白丝氨酸（1756位）附近的残基一样。该位点相当于大鼠IP3 受体序列中的Ser1756.肌醇1,4,5 - 三磷酸受体，也被称为IP3R 或InsP3R，是细胞内钙释放通道家族的成员，位于内质网中。IP3R可作为Ca2 +细胞内储存的钙离子释放通道的功能。有三种IP3受体类型（IP3R1，2，和3），第二信使激活肌醇1,4,5 - 三磷酸（IP3）（1）。四个独立的亚基同源或异源寡聚体化以形成受体的功能通道（2）。IP3R1丝氨酸（1756位）磷酸化，通过环腺苷酸AMP依赖性蛋白激酶（PKA）调节IP3R1至IP3的灵敏性，可能作为Ca2 +释放调节模型(3,4)。IP3R1介导的Ca2 +释放似乎对蒲金耶氏细胞长时程抑制（LTD）的诱导有影响（5）。

1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

原厂资料：

Specificity / Sensitivity

Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb detects endogenous levels of IP3 receptor only when phosphorylated at Ser1756.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1764 of human IP3 receptor protein. This site is equivalent to Ser1756 in the rat IP3 receptor sequence.

Background

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. IP3R functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2, and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual subunits homo- or hetero-oligomerize to form the receptor's functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may be a mode of regulation for Ca2+ release (3,4). IP3R1-mediated Ca2+ release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).

1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.