ATPIF1 Antibody

• 产品编号：CST-8528S      品牌：CST       原厂货号：8528S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ATPIF1抗体识别内源性的ATPIF1总蛋白水平。该多克隆抗体用与人类ATPIF1中Ala37位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化制得。TP酶抑制因子1（ATPIF1）基因编码线粒体ATP酶抑制剂，该抑制剂可限制线粒体呼吸受损时ATP耗竭（1）。ATPIF1在pH降低时被激活，结合β-F1-ATPase，抑制H-ATP合成酶的水解活性（1,2）。ATPIF1除了作为一个ATP水解酶的作用，也被证明在细胞能量代谢中发挥调节作用，它可以引发癌细胞的有氧糖酵解导致其Warburg表型（3,4）。在几个人类肿瘤中过度表达的ATPIF1进一步支持其参与肿瘤发生，并改变了癌细胞的新陈代谢，包括重编程能量代谢模式至糖酵解（3）。

1. Gledhill, J.R. et al. (2007) Proc Natl Acad Sci USA 104, 15671-6.
2. Cabezón, E. et al. (2003) Nat Struct Biol 10, 744-50.
3. Sánchez-Cenizo, L. et al. (2010) J Biol Chem 285, 25308-13.
4. Wallace, D.C. (2005) Cold Spring Harb Symp Quant Biol 70, 363-74.

原厂资料：

Specificity / Sensitivity

ATPIF1 Antibody recognizes endogenous levels of total ATPIF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala37 of human ATPIF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The ATPase inhibitor factor 1 (ATPIF1) gene encodes a mitochondrial ATPase inhibitor that limits ATP depletion when mitochondrial respiration is impaired (1). ATPIF1 becomes activated following a drop in pH, binding to β-F1-ATPase, thereby inhibiting the hydrolase activity of the H+-ATP synthase (1,2). In addition to its role as an ATP hydrolase, ATPIF1 has also been shown to play a regulatory role in cellular energy metabolism by triggering the induction of aerobic glycolysis in cancer cells resulting in their Warburg phenotype (3,4). The overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis (3).

1. Gledhill, J.R. et al. (2007) Proc Natl Acad Sci USA 104, 15671-6.
2. Cabezón, E. et al. (2003) Nat Struct Biol 10, 744-50.
3. Sánchez-Cenizo, L. et al. (2010) J Biol Chem 285, 25308-13.
4. Wallace, D.C. (2005) Cold Spring Harb Symp Quant Biol 70, 363-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.