BAG6 Antibody

• 产品编号：CST-8523S      品牌：CST       原厂货号：8523S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BAG6 Antibody识别内源性的BAG6总蛋白。它与其它BCL2相关athanogene（BAG）家族成员无交叉反应。该多克隆抗体用与人类BAG6蛋白羧基末端附近的氨基酸链对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。BAG6（BCL2相关athanogege-6）也称为BAT3（HLA-B相关转录本3），在人主要组织相容性复合物中III类区域中发现的基因，之后发现它具有蛋白质分子伴侣的活性。BAG6，与其它分子伴侣和泛素连接酶结合，调节蛋白质稳定性，将尾端锚定的膜蛋白插入到内质网上（1-3）。BAT3复合物，由BAG6、TRC35和Ub14a组成，其定位于正在合成膜蛋白的核糖体，促进尾端锚定蛋白被TRC40捕获，并将新生蛋白质插入内质网膜（4，5）。BAG6也在通过内质网相关的降解和泛素蛋白酶体系统进行的错误折叠或错误定位肽段的清除中扮演重要作用（1，6，7）。BAG6也能够通过其与DERLIN2相互作用扮演糖蛋白的分子伴侣作用（8）。除了分子伴侣，BAG6还与调节染色质结构和基因表达有关。例如，BAG6和SET1A作为BORIS的结合伴侣，作用并影响染色质结构和基因表达（9）。类似的，增加BAG6的表达能够诱导p300介导的p53乙酰化，这一过程被DNA损伤反应所需（10）。BAG6还被发现可与TGF-β相互作用，作为TGF-β1的刺激1型胶原表达的正向调节因子（11）。BAG6也能够通过与小C末端结构域磷酸酶（SCP）相互作用去磷酸化SMAD蛋白质，导致BMP介导事件的终结，从而参与抑制骨形态发生蛋白（BMP）信号通路（12）。

1. Hessa, T. et al. (2011) Nature 475, 394-7.
2. David, R. (2011) Nat Rev Mol Cell Biol 12, 550.
3. Ast, T. and Schuldiner, M. (2011) Curr Biol 21, R692-5.
4. Mariappan, M. et al. (2010) Nature 466, 1120-4.
5. Leznicki, P. et al. (2010) J Cell Sci 123, 2170-8.
6. Minami, R. et al. (2010) J Cell Biol 190, 637-50.
7. Wang, Q. et al. (2011) Mol Cell 42, 758-70.
8. Claessen, J.H. and Ploegh, H.L. (2011) PLoS One 6, e28542.
9. Nguyen, P. et al. (2008) Mol Cell Biol 28, 6720-9.
10. Sasaki, T. et al. (2007) Genes Dev 21, 848-61.
11. Kwak, J.H. et al. (2008) J Biol Chem 283, 19816-25.
12. Goto, K. et al. (2011) Cell Death Dis 2, e236.

原厂资料：

Specificity / Sensitivity

BAG6 Antibody recognizes endogenous levels of total BAG6 protein. It does not cross-react with other BCL2-associated athanogene (Bag) family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BAG6 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

BAG6 (BCL2-associated athanogene-6), alternately known as BAT3 (HLA-B-associated transcript 3), was originally identified as a gene within the class III region of the human major histocompatibility complex, but has subsequently been found to exhibit protein chaperone activity. BAG6, in conjunction with other chaperone proteins and ubiquitin ligases, regulates protein stability and insertion of tail-anchored membrane proteins into the endoplasmic reticulum (1-3). The BAT3 complex, consisting of BAG6, TRC35 and Ubl4a localizes to ribosomes synthesizing membrane proteins and facilitates tailed-anchored protein capture by TRC40 and subsequent insertion of the nascent protein in to the ER membrane (4,5). BAG6 also plays a critical role in clearing cells of mis-folded and mis-localized peptides via endoplasmic reticulum-associated degradation and the ubiquitin-proteasome system (1,6,7). BAG6 may also act as a chaperone for glycoproteins through its interaction with DERLIN2 (8). 
 In addition to its role as a chaperone, BAG6 has also been implicated in regulating chromatin structure and gene expression. For example, BAG6 and SET1A act as binding partners for BORIS to effect changes of chromatin structure and gene expression (9). Similarly, increased expression of BAG6 induces p300-mediated acetylation of p53, which is required for DNA damage response (10). BAG6 has also been found to interact with TGF-β, and in so doing acts as a positive regulator of TGF-β1 stimulation of type 1 collagen expression (11). BAG6 also suppresses bone morphogenic protein (BMP) signaling via its interaction with and regulation of small C-terminal domain phosphatase (SCP) that dephosphorylates SMAD proteins resulting in subsequent termination of BMP-mediated events (12).

1. Hessa, T. et al. (2011) Nature 475, 394-7.
2. David, R. (2011) Nat Rev Mol Cell Biol 12, 550.
3. Ast, T. and Schuldiner, M. (2011) Curr Biol 21, R692-5.
4. Mariappan, M. et al. (2010) Nature 466, 1120-4.
5. Leznicki, P. et al. (2010) J Cell Sci 123, 2170-8.
6. Minami, R. et al. (2010) J Cell Biol 190, 637-50.
7. Wang, Q. et al. (2011) Mol Cell 42, 758-70.
8. Claessen, J.H. and Ploegh, H.L. (2011) PLoS One 6, e28542.
9. Nguyen, P. et al. (2008) Mol Cell Biol 28, 6720-9.
10. Sasaki, T. et al. (2007) Genes Dev 21, 848-61.
11. Kwak, J.H. et al. (2008) J Biol Chem 283, 19816-25.
12. Goto, K. et al. (2011) Cell Death Dis 2, e236.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody