Caveolin-2 (D4A6) XP? Rabbit mAb

• 产品编号：CST-8522S      品牌：CST       原厂货号：8522S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caveolin-2 (D4A6) XP® Rabbit mAb可以识别内源性总的小窝蛋白-2.基于蛋白的氨基酸序列，该抗体与caveolin-1或caveolin-3没有交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段与人小窝蛋白氨基端附近氨基酸一致。21-24kDa整合素相关蛋白——小窝蛋白，是胆固醇/富含鞘脂质膜微区小窝蛋白的主要结构部件。已经发现小窝蛋白家族的3个成员（caveolin-1, -2,和-3）有不同的组织分布。小窝蛋白会形成异源或同源寡聚物与胆固醇和其他脂质结合（1）。小窝蛋白涉及了不同的生物功能，包括囊泡转运，胆固醇动态平衡，细胞粘附，细胞凋亡，也牵涉到到神经退行性疾病（2）。小窝蛋白和多种信号分子相互作用，例如Gα亚基，酪氨酸激酶受体，PKCs，Src家族酪氨酸激酶和eNOS (1,2).据认为小窝蛋白作为信号转导整合的支架蛋白。第14位酪氨酸的磷酸化对小窝蛋白与含有SH2或PTB结构域的受体蛋白的结合是至关重要的，例如GRB7（3-5）。第80位丝氨酸的磷酸化调控小窝蛋白与内质网膜结合并进入分泌通路（6）。

1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

原厂资料：

Specificity / Sensitivity

Caveolin-2 (D4A6) XP® Rabbit mAb recognizes endogenous levels of total caveolin-2 protein. Based on amino acid sequence, the antibody is not expected to cross-react with caveolin-1 or caveolin-3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human caveolin protein.

Background

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).

1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody