Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP? Rabbit mAb (Pacific Blue? Conjugate)

• 产品编号：CST-8520S      品牌：CST       原厂货号：8520S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb 兔单抗(Pacific Blue™ 接合的)能够检测仅在Ser235和 Ser236位点磷酸化的内源性核糖体蛋白S6的水平。该单克隆抗体是经由合成的对应于人核糖体蛋白S6的 Ser235 和Ser236位点附近序列的磷酸化肽段免疫动物而生产的。该CST抗体偶联了Pacific Blue™荧光染料。并通过直标法流式细胞术在人细胞内进行内部检测。它与未接合的Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 #4858具有相同的种属交叉反应性。生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关，而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Pig

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and Ser236.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2mg/ml BSA. Store at 4°C. Do not aliquot the antibody.
Protect from light. Do not freeze.