Caspase-14 Antibody

• 产品编号：CST-8519S      品牌：CST       原厂货号：8519S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caspase-14 Antibody能够检测内源性水平caspase-14总蛋白和成熟caspase-14的p11亚基。该多克隆抗体是由合成的人源caspase-14蛋白C末端肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。Caspases是半胱氨酸蛋白酶家族的一员，在执行细胞凋亡中起着至关重要的作用。Caspase-14，也叫MICE，是一个独特的限制性表达的caspase家族成员，它在胚胎组织和成人的皮肤中被发现（1,2）。Caspase-14被caspase-8轻微加工成p18和p11亚基（2）。Caspase-14可能不在细胞凋亡中发挥作用（2），而是可以调控角化细胞的分化（3）。Caspase-14的表达可保护避免牛皮癣（4,5）和辐照损伤（6）。Caspase-14也可能在角质细胞的终末分化时中间丝相关蛋白的蛋白水解加工过程起作用（6）。

1. Hu, S. et al. (1998) J Biol Chem 273, 29648-53.
2. Van de Craen, M. et al. (1998) Cell Death Differ 5, 838-46.
3. Eckhart, L. et al. (2000) Biochem Biophys Res Commun 277, 655-9.
4. Lippens, S. et al. (2004) Am J Pathol 165, 833-41.
5. Walsh, D.S. et al. (2005) J Dermatol Sci 37, 61-3.
6. Denecker, G. et al. (2007) Nat Cell Biol 9, 666-74.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Caspase-14 Antibody recognizes endogenous levels of total caspase-14 protein and the p11 subunit of mature caspase-14.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caspase-14 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspases are a family of cysteine proteases that play an essential role in carrying out apoptosis. Caspase-14, also named MICE, is a unique member of the caspase family with restricted expression; it is found in embryonic tissues and adult skin (1,2). Caspase-14 is weakly processed into p18 and p11 subunits by caspase-8 (2). Caspase-14 may not play a role in apoptosis (2), but instead may regulate keratinocyte differentiation (3). Expression of caspase-14 may protect from psoriasis (4,5) and irradiation damage (6). Caspase-14 may also be responsible for proteolytic processing of filaggrin during terminal differentiation of keratinocytes (6).

1. Hu, S. et al. (1998) J Biol Chem 273, 29648-53.
2. Van de Craen, M. et al. (1998) Cell Death Differ 5, 838-46.
3. Eckhart, L. et al. (2000) Biochem Biophys Res Commun 277, 655-9.
4. Lippens, S. et al. (2004) Am J Pathol 165, 833-41.
5. Walsh, D.S. et al. (2005) J Dermatol Sci 37, 61-3.
6. Denecker, G. et al. (2007) Nat Cell Biol 9, 666-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody