Insulin (C27C9) Rabbit mAb (PE Conjugate)

• 产品编号：CST-8508S      品牌：CST       原厂货号：8508S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Insulin (C27C9)兔单克隆抗体（PE结合）识别内源性的insulin总蛋白水平。该单克隆抗体用与人类insulin蛋白氨基酸序列对应的人工合成肽段免疫动物制成。CST公司生产的抗体在与phycoerythrin (PE)结合，已通过在人和小鼠细胞上直接进行流式细胞仪分析的内部测试。。该抗体预计可与未结合的Insulin (C27C9) Rabbit mAb兔单抗 #3014发生同种交叉反应。血糖平衡是一个被激素调节的核心生理过程。摄食后血糖水平升高，刺激胰岛素通过血糖敏感的途径从胰岛β细胞中释放（1）。胰岛素在分泌前先合成为前胰岛素。A和B链通过二硫键结合形成胰岛素，前体分子的中心区域被切除形成C肽。胰岛素刺激葡萄糖从血中被摄取进入骨骼肌和脂肪组织。胰岛素缺乏导致1型糖尿病（2）。

1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
2. Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.

原厂资料：

Specificity / Sensitivity

Insulin (C27C9) Rabbit mAb (PE Conjugate) detects endogenous levels of total insulin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Insulin (C27C9) Rabbit mAb #3014.

Background

The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).

1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
2. Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2mg/ml BSA. Store at 4°C. Do not aliquot the antibody.
Protect from light. Do not freeze.