Nestin (Rat-401) Mouse mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-8489S      品牌：Cell Signaling Technology       原厂货号：8489S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

该单克隆抗体用大鼠脊髓E15免疫动物而制备的。这款CST抗体标记了Alexa Fluor® 647 荧光染料，通过了内部测试可以直接用于大鼠大脑的免疫荧光试验。这款抗体与不带标记的Nestin (Rat-401) Mouse mAb 鼠单抗 #4760有着相同的种属交叉反应。巢蛋白是中间纤维家族的蛋白，与神经纤维蛋白结构上相关（1）。在发育的大脑中高表达，它可以调节细胞结构和细胞分裂及迁移所涉及的胞内过程（1,2）。大脑成熟后，巢蛋白的表达迅速下调，被神经纤维蛋白的表达所替代（2）。因为在成熟神经元，前体神经元和神经胶质细胞都有表达，在发育中的大脑，损伤后的大脑与脊髓中也有表达，巢蛋白作为神经干/祖细胞的标记已经得到了广泛的认可（3）。巢蛋白的表达也在多种神经肿瘤中被发现，包括胶质瘤、神经纤维瘤、星形细胞瘤，因此它作为神经肿瘤干细胞的标记也受到了认可（3）。研究发现巢蛋白也在星形细胞、视网膜、心肌、胰腺和其它组织中表达中（3）。所以，目前关于巢蛋白是神经干/祖细胞的唯一标志这一说法尚未得到大家一致肯定。

1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
2. Giliarov, A.V. (2007) Morfologiia 131, 85-90.
3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.

原厂资料：

Specificity / Sensitivity

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of nestin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for immunofluorescent analysis in rat brain. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nestin (Rat-401) Mouse mAb #4760.

Background

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
2. Giliarov, A.V. (2007) Morfologiia 131, 85-90.
3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze.