Phospho-Catenin δ-1 (Ser252) Antibody

• 产品编号：CST-8477S      品牌：CST       原厂货号：8477S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化Catenin δ-1（丝氨酸252）抗体仅仅在252位丝氨酸被磷酸化时识别内源性Catenin δ-1水平。多抗由合成的磷酸化肽段免疫动物产生，该合成肽段对应人Catenin δ-1蛋白的252位丝氨酸附近残基。抗体由蛋白A和肽段亲和层析技术纯化。Catenin δ-1 (p120 catenin)有一个N氨基末端螺旋式盘旋结构域，紧跟着一个含多个磷酸化位点的调控结构域，和一个中心Armadillo重复结构域，该结构域是十个连锁的42氨基酸重复单元。C末端尾为未知功能（1）。Catenin δ-1在细胞间粘附调控履行重要作用，它在细胞表面调控E-cadherin更新，决定能对细胞粘附起作用的E-cadherin水平（2）。Catenin δ-1对cadherin调节的粘附有正向和负向效应（3）。肌动蛋白动力学机制也被catenin δ-1调控，catenin δ-1能调控RhoA, Rac, 和cdc42蛋白（1）。类似于β-catenin，catenin δ-1转位入核，虽然这种定位的作用未知，许多研究显示catenin δ-1不规则表达，或在各种类型肿瘤细胞中缺乏，暗示catenin δ-1可能作为肿瘤抑制因子其功能（4）。Catenin δ-1在丝氨酸252位点的磷酸化由Cell Signaling Technology (CST)通过PhosphoScan®这种CST™ LC-MS/MS平台发现磷酸化位点（5）。

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-Catenin δ-1 (Ser252) Antibody recognizes endogenous levels of Catenin δ-1 protein only when phosphorylated at Ser252.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser252 of human Catenin δ-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).Phosphorylation of Ser252 on Catenin δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (5).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.