Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb

• 产品编号：CST-8467S      品牌：CST       原厂货号：8467S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化 Zyxin (丝氨酸142/143) (D1E8)兔源单抗仅仅在丝氨酸142和丝氨酸143 磷酸化时识别内源性Zyxin 蛋白水平。单抗由合成肽段免疫动物产生，该合成肽段对应人zyxin蛋白的142位和143位丝氨酸附近残基。Zyxin家族蛋白包括LIMD1, ajuba, trip6和zyxin,每个都在C末端含三个LIM结构域。Zyxin家族成员与肌动蛋白细胞骨架有关，是细胞连接粘附复合物和整合素调控粘附复合物的元件。它们在细胞核内外穿梭，细胞核内它们可能在转录激活中起功能(1)。Zyxin涉及在焦点粘连处机械力诱导的肌动蛋白聚合的调控（2）。在粘附、迁移调控中， 很可能通过招募Ena/VASP蛋白到焦点粘附中（3）。Zyxin与肿瘤抑制因子myopodin相互作用或调控它的功能，myopodin抑制肿瘤生长和转移（4）Zyxin可能被Akt磷酸化142位丝氨酸（5）。

1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
2. Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
3. Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
4. Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.

原厂资料：

Specificity / Sensitivity

Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142 and Ser143.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 and Ser143 of human zyxin protein.

Background

The zyxin family of proteins includes LIMD1, ajuba, trip6, and zyxin, each of which contains three LIM domains at the carboxy terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2) and in the regulation of adhesion and migration, possibly through the recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Zyxin may be phosphorylated by Akt at Ser142 (5).

1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
2. Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
3. Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
4. Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody