Human His64-1BB?Ligand/TNFSF9 (hHis64-1BBL)

• 产品编号：CST-8460LF      品牌：CST       原厂货号：8460LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TNF Family 肿瘤坏死因子
• 应用分类：

描述：

CST公司利用重组的人His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802)在人的293细胞中表达。重组的h4-1BBL蛋白在N-末端有His6标签，其分子量据推算为22,020Da。DTT-还原和未还原的蛋白迁移时是作为一个22kDa的多肽而转移。重组h4-1BBL蛋白的N-末端序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) h_His64-1BBL通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白h_His64-1BBL的生物活性是通过其在功能性ELISA实验中对4-1BB的结合能力来确定的。每个批次的ED50在6-18 ng/ml之间。内毒素含量:<0.01 ng /1 μg h_His64-1BBL。有载体: 每微克h_His64-1BBL蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。4-1BBL(也叫做TNFSF9)是TNF-R超家族中的一员，此家族包括OX40L, CD27L, CD40L和GITRL(1)。4-1BBL主要在具有抗原的细胞中表达，比如巨噬细胞、树突状细胞和B细胞(2)。4-1BBL结合到4-1BB的表面并激活T细胞(1)。4-1BBL/4-1BB结合后通过TRAF1, 2和 3而激活 NF-κB信号通路(1)。4-1BBL作为供刺激因子分子，诱导T下表增殖、细胞溶解活性和细胞因子的产生(2)。基质金属蛋白能通过将其从细胞表面的剪切而引起4-1BBL的可溶性(3)。可溶性4-1BBL在体外的活性能通过结合而增强(4,5)。

原厂资料：

Source / Purification

Recombinant human His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged h4-1BBL has a calculated MW of 22,020. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides. The expected amino terminus of recombinant hHis64-1BBL was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis64-1BBL. All lots are greater than 98% pure.

Bioactivity

The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 6-18 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis64-1BBL.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis64-1BBL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

4-1BBL (also known as TNFSF9) is a member of the TNF-R superfamily, which includes OX40L, CD27L, CD40L, and GITRL (1). 4-1BBL is expressed primarily on antigen presenting cells, such as macrophages, dendritic cells, and B cells (2). 4-1BBL binds to 4-1BB on the surface of activated T cells (1). 4-1BBL/4-1BB binding activates the NF-κB pathway via TRAF1, 2, and 3 (1). 4-1BBL functions as a co-stimulatory molecule, inducing T cell proliferation, cytolytic activity, and cytokine production (2). Matrix metalloproteases can generate soluble 4-1BBL by cleaving it from the cell surface (3). The in vitro activity of soluble 4-1BBL is enhanced by cross-linking (4,5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.