IFN-γ (D3H2) XP? Rabbit mAb

• 产品编号：CST-8455P      品牌：Cell Signaling Technology       原厂货号：8455P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IFN-γ (D3H2) XP® Rabbit mAb兔单抗检测内源性IFN-γ总蛋白水平。此单克隆抗体通过重组人源IFN-γ蛋白免疫动物制备。IFN-γ在先天免疫和获得性免疫应答中发挥重要作用。IFN-γ激活先天免疫细胞如巨噬细胞和NK细胞的细胞毒活性(1,2)。通过NK细胞和具有抗原细胞(APCs)产生IFN-γ促进了细胞介导的获得性免疫，包括诱导T淋巴细胞产生IFN-γ、增加I型和II型 MHC 分子的表达、增强肽抗原呈递(1)。IFN-γ的抗病毒活性与PKR蛋白的诱导和其它的调控蛋白有关。IFN-γ结合到 IFNGR1/IFNGR2 复合体促进了受体的二聚化并形成(IFNGR1/IFNGR2)2 -IFN-γ二聚体。这种结合诱导了受体胞内域构象的改变并激活包括Jak1、 Jak2和 Stat1 的信号通路(3)。IFN-γ的重要作用是放大免疫监控，在IFN-γ或IFNGR敲除老鼠和IFNGR1或IFNGR2失活突变体中，病原菌感染的易感性增加。IFN-γ在动脉硬化中也发挥了重要的作用(4)。

1. Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
2. Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
3. Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
4. McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.

原厂资料：

Specificity / Sensitivity

IFN-γ (D3H2) XP® Rabbit mAb recognizes endogenous levels of total IFN-γ protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IFN-γ protein.

Background

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

1. Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
2. Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
3. Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
4. McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.