Phospho-NDRG1 (Thr346) (D98G11) XP? Rabbit mAb (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-8453S      品牌：CST       原厂货号：8453S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb (Alexa Fluor^® 594 偶联)识别Thr346被磷酸化的内源性NDRG1蛋白。此抗体似也识别NDRG1的保守磷酸化位点，如Thr356 和 Thr366。此单克隆抗体由合成肽段免疫动物产生，该肽段与人NDRG1蛋白Thr346邻近氨基酸残基序列一致。此Cell Signaling Technology公司的抗体偶联了Alexa Fluor^® 594荧光染料在公司内部测试了人和小鼠细胞的免疫荧光分析。预测此抗体与未偶联的Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb #5482有相同的种属交叉反应。N-myc下游调控gene1（NDRG1），也被称为Cap43，Drg1，RTP/rit42和Proxy-1，是NDRG家族的一员，该家族包含的4个成员（NDRG1-4）在生长，分化和细胞存活过程中发挥作用（1-5）。广泛表达的NDRG1并对多种压力信号发生应激反应，包括DNA损伤（4），缺氧（5），和镍及钙浓度升高（2）。NDRG1在N-myc缺陷小鼠中表达升高，能够被N-和c-myc负调控（1-6）。在DNA损伤过程中，NDRG1通过p53信号激活，并且是p53介导的凋亡过程中所必须的（4,7）。研究显示，NDRG1通过促进分化，抑制生长，调控血管生成和肿瘤转移等进程中发挥功能（3,4,6,8,9）。NDRG1基因发生无义突变后会引发遗传性运动和感觉神经病病变-Lom(HMSNL),该结论由证明NDRG1在维持髓鞘和轴突存活的研究支持（10,11）。NDRG1在肥大细胞成熟过程中表达上调，它的缺失会导致衰减的过敏反应（12）。NDRG1和NDRG2都是SGK1的底物，尽管SGK1介导的磷酸化的生理性作用尚未可知（13）。NDRG1可以被SGK1在Thr328, Ser330, Thr346, Thr356,以及Thr366磷酸化。NDRG1被SGK1磷酸化后可以被GSK-3磷酸化。

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

原厂资料：

Specificity / Sensitivity

Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of NDRG1 when phosphorylated at Thr346. This antibody likely cross-reacts with other conserved phosphorylation sites on NDRG1, such as Thr356 and Thr366.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr346 of mouse NDRG1 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human and mouse cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb #5482.

Background

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze.