INPP4b (D19B9) Rabbit mAb

• 产品编号：CST-8450S      品牌：CST       原厂货号：8450S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

INPP4b(D19B9)的兔单克隆抗体识别内源性的全部INPP4b蛋白。该单克隆抗体用与人类INPP4b蛋白中羧基末端附近的氨基酸序列对应的重组蛋白免疫动物而制成。磷酯酰肌醇脂和磷酸肌醇是重要的第二信使，它们的产生控制着许多细胞活动。这些分子的细胞内水平受磷酸肌醇激酶和磷酸酶调节。目前已知最全面的脂质激酶之一即是磷酸肌醇3激酶（PI3K），它负责酰肌醇头D-3位的磷酸化（1）。PI3K催化磷酸肌醇（PI）、4-磷酸磷酸肌醇（PIP）和4,5-二磷酸磷酸肌醇磷酸化产生3,4,5-三磷酸磷酸肌醇。生长因子和激素可引发这种磷酸化事件，这反过来又协调细胞生长、细胞周期起始、细胞迁移和细胞生存（1）。PTEN基因是目前了解得比较清楚的伙伴磷酸酶，可以从PI（3,4,5）P3的D-3位置上去除磷酸基产生PI（4,5）P2（1,2）。SHIP1或SHIP2对D-5位的去磷酸化可产生PI（3,4）P2（3），肌醇磷酸酶4-磷酸酶同工酶I（INPP4a）和同工酶II（INPP4b）对D-4位去磷酸化可产生PI(3)P（4,5）。虽然INPP4a已与神经细胞的生存和巨核细胞谱系决定有关（6,7），但对INPP4b仍然知之甚少。它已被证明两个INPP4b的剪接体发生在小鼠中，每个变体均显示出不同的组织分布和亚细胞定位（5,8）。

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
3. Ware, M.D. et al. (1996) Blood 88, 2833-40.
4. Norris, F.A. et al. (1995) J Biol Chem 270, 16128-33.
5. Norris, F.A. et al. (1997) J Biol Chem 272, 23859-64.
6. Nystuen, A. et al. (2001) Neuron 32, 203-12.
7. Vyas, P. et al. (2000) Proc Natl Acad Sci USA 97, 13696-701.
8. Ferron, M. and Vacher, J. (2006) Gene 376, 152-61.

原厂资料：

Specificity / Sensitivity

INPP4b (D19B9) Rabbit mAb recognizes endogenous levels of total INPP4b protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human INPP4b protein.

Background

Phosphotidylinositol lipids and phosphoinositides are important second messengers, their generation controlling many cellular events. Intracellular levels of these molecules are regulated by phosphoinositide kinases and phosphatases. One of the best characterized lipid kinases is phosphoinositide 3-kinase (PI3K), which is responsible for phosphorylation on the D-3 position of the inositide head group (1). This action of PI3K catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN, the well characterized partnering phosphatase, reverses this process by removing the phosphate from PI(3,4,5)P3 at the D-3 position to generate PI(4,5)P2 (1,2). Dephosphorylation on the D-5 position to generate PI(3,4)P2 occurs through the action of SHIP1 or SHIP2 (3), and dephosphorylation on the D-4 position to generate PI(3)P can occur through the action of inositol polyphosphate 4-phosphatase isoenzymes type I (INPP4a) and type II (INPP4b) (4,5). While INPP4a has been implicated in neuronal survival and megakaryocyte lineage determination (6,7), less is understood about INPP4b. It has been shown that two splice variants of INPP4b occur in mice, each showing distinct tissue distribution and subcellular localization (5,8).

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
3. Ware, M.D. et al. (1996) Blood 88, 2833-40.
4. Norris, F.A. et al. (1995) J Biol Chem 270, 16128-33.
5. Norris, F.A. et al. (1997) J Biol Chem 272, 23859-64.
6. Nystuen, A. et al. (2001) Neuron 32, 203-12.
7. Vyas, P. et al. (2000) Proc Natl Acad Sci USA 97, 13696-701.
8. Ferron, M. and Vacher, J. (2006) Gene 376, 152-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.