StAR (D10H12) XP? Rabbit mAb

• 产品编号：CST-8449S      品牌：CST       原厂货号：8449S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

StAR (D10H12) XP® Rabbit mAb兔单抗用于检测内源性StAR总蛋白水平。该单抗通过用与人源StAR蛋白Arg272位点周围残基序列相同的合成肽免疫动物制备而得。类固醇生成快速调节蛋白(StAR)在胆固醇从质膜外膜转运到线粒体内膜的过程中起着重要作用(1)。它的前体分子量的37 kDa，经剪切生成分子量为28kDa活性蛋白，活性蛋白可以将胆固醇代谢生成孕烯醇酮(2,3)。StAR主要在分泌类固醇的肾上腺和性腺组织中表达(3)。在常染色体先天性肾上腺增生(LCAH)中，常见StAR的异常表达，它的异常表达会导致孕烯醇酮和皮质醇代谢的紊乱(4)。到目前为止，胆固醇结合到StAR上的机制还不清楚(4)。

1. Caron, K.M. et al. (1997) Proc Natl Acad Sci U S A 94, 11540-5.
2. Bose, H.S. et al. (1999) Proc Natl Acad Sci U S A 96, 7250-5.
3. Manna, P.R. et al. (2002) Biol Reprod 67, 1393-404.
4. Roostaee, A. et al. (2008) Biochem J 412, 553-62.

原厂资料：

Specificity / Sensitivity

StAR (D10H12) XP® Rabbit mAb recognizes endogenous levels of total StAR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg272 of human StAR protein.

Background

Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane (1). The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone (2,3). StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue (3). Abnormalities in StAR gene expression are impacted in autosomal Lipoid Congenial Adrenal Hyperplasia (LCAH) resulting in defects in pregnenolone and cortisol synthesis (4). The mechanism of cholesterol binding to StAR has yet to be elucidated (4).

1. Caron, K.M. et al. (1997) Proc Natl Acad Sci U S A 94, 11540-5.
2. Bose, H.S. et al. (1999) Proc Natl Acad Sci U S A 96, 7250-5.
3. Manna, P.R. et al. (2002) Biol Reprod 67, 1393-404.
4. Roostaee, A. et al. (2008) Biochem J 412, 553-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.