Human C-C Motif Chemokine 3 (hCCL3/MIP-1-α)

• 产品编号：CST-8445SC      品牌：CST       原厂货号：8445SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 趋化因子
• 应用分类：

描述：

CST在大肠杆菌细胞中表达重组的人CCL3 (hCCL3) Ala27-Ala92 (Accession #NP_002974)蛋白。重组的hCCL3蛋白在N-末端不含有标签，其分子量据推算为7445Da。DTT-还原和未还原的蛋白迁移时是作为一个8kDa的多肽而转移。重组hCCL3蛋白的N-末端ADTPT的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) 重组hCCL通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hCCL3的生物活性是通过THP-1细胞的迁移实验确定的。每个批次的ED50在0.5-1.2 ng/ml之间。每微克hCCL3内毒素含量小于0.01 ng。

有载体: 每微克hCCL3蛋白溶解在包含100 mM NaCl和20 μg BSA的20 mM 柠檬酸盐(pH 3.0)的溶液中，通过 0.22 μm 滤膜并冻干。

无载体：每微克hCCL3蛋白溶解在包含100 mM NaCl的20 mM 柠檬酸盐(pH 3.0)的溶液中，通过 0.22 μm 滤膜并冻干。

CCL3, 也叫做 MIP-1-α或 LD78α, 是定位于膜上的8 kDa C-C家族趋化因子，它在免疫监控、炎症和感染等过程发挥了重要的作用(1)。CCL3与CCL4 (MIP-1-β)有68%氨基酸相似度与LD78β具有94%的氨基酸相似度(1)。CCL3的产生是通过激活的单核细胞、激活的巨噬体、NK细胞、T细胞以及其他的细胞类型(1)。CCL3有两个受体: CCR1和CCR5 (1)。CCL3通过与R5 HIV-1病毒竞争其共受体 CCR5 而抑制病毒的感染(1)。CCL3的多态性与类风湿性关节炎和溃疡性结肠炎的发展有关(2,3)。

原厂资料：

Source / Purification

Recombinant human CCL3 (hCCL3) Ala27-Ala92 (Accession #NP_002974) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hCCL3 does not have a Met on the amino terminus and has a calculated MW of 7445. DTT-reduced and non-reduced protein migrate as 8 kDa polypeptides. The expected amino-terminal ADTPT of recombinant hCCL3 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCCL3. All lots are greater than 98% pure.

Bioactivity

The activity of hCCL3 was determined using a THP-1 cell migration assay. The ED50 of each lot is between 0.5-1.2 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hCCL3.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hCCL3. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

CCL3, also know as MIP-1-α, or LD78α, is an 8 kDa member of the C-C family of chemokines, which play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1-β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells among others (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (2,3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.