Integrin α4 (D2E1) XP? Rabbit mAb

• 产品编号：CST-8440P      品牌：Cell Signaling Technology       原厂货号：8440P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Integrin α4 (D2E1) XP® Rabbit mAb用于检测内源性的整合素α4总蛋白。该单克隆抗体由α4整合素第1027位丝氨酸周围的合成多肽免疫动物产生。整合素是细胞表面的α/β异二聚体受体，在细胞粘附、迁移、生长和存活中均起关键作用(1,2)。至少18种α亚基和8种β亚基，形成24种已知的整合素分布在不同的组织并具有交叉的配体特异性(3)。整合素不仅对细胞外环境信号应答并给细胞传输信号（从外向内传导），而且感知细胞内环境从而改变与细胞外环境的相互作用（由内向外传导）(1,2)。一对重要的α4整合素，α4β1 和α4β7，在细胞粘附处与VCAM-1、纤连蛋白以及MAdCAM-1相互作用(3)。基因敲除及抗体封闭试验显示α4整合素在胚胎肝脏和心脏发育及胎儿淋巴细胞归巢中起重要作用(4-6)。α4整合素胞质尾区988位丝氨酸的磷酸化阻止了桩蛋白的结合并促进了主要的极化迁移(7,8)。在SDS-PAGE上，α4整合素分布在几个不同的分子大小位置，150 kDa的成熟蛋白和140 kDa的前体蛋白（在温和的条件下180 kDa的蛋白也可以出现）(9)。α4整合素在588位精氨酸有1个切割位点，从而形成80 kDa的N-末端或70 kDa的C-末端片段(10)。

1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
2. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
3. Plow, E.F. et al. (2000) J Biol Chem 275, 21785-8.
4. Bonder, C.S. et al. (2005) Immunity 23, 153-63.
5. Arroyo, A.G. et al. (1999) Immunity 11, 555-66.
6. Yang, J.T. et al. (1995) Development 121, 549-60.
7. Nishiya, N. et al. (2005) Nat Cell Biol 7, 343-52.
8. Alon, R. et al. (2005) J Cell Biol 171, 1073-84.
9. Teixidó, J. et al. (1992) J Biol Chem 267, 1786-91.
10. Pujades, C. et al. (1996) Biochem J 313 ( Pt 3), 899-908.

原厂资料：

Specificity / Sensitivity

Integrin α4 (D2E1) XP® Rabbit mAb recognizes endogenous levels of total integrin α4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1027 of human integrin α4 protein.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). 
 A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks binding to paxillin and promotes leading edge migration (7,8). 
 On SDS-PAGE, integrin α4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin α4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).

1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
2. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
3. Plow, E.F. et al. (2000) J Biol Chem 275, 21785-8.
4. Bonder, C.S. et al. (2005) Immunity 23, 153-63.
5. Arroyo, A.G. et al. (1999) Immunity 11, 555-66.
6. Yang, J.T. et al. (1995) Development 121, 549-60.
7. Nishiya, N. et al. (2005) Nat Cell Biol 7, 343-52.
8. Alon, R. et al. (2005) J Cell Biol 171, 1073-84.
9. Teixidó, J. et al. (1992) J Biol Chem 267, 1786-91.
10. Pujades, C. et al. (1996) Biochem J 313 ( Pt 3), 899-908.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody