Human Transforming Growth Factor β3 (hTGF-β3)

• 产品编号：CST-8425LC      品牌：CST       原厂货号：8425LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TGF-beta 转化生长因子beta超家族
• 应用分类：

描述：

Cell Signaling Technology公司重组人源TGF-β3丙氨酸301-丝氨酸412（编号NP_003230），在人源293细胞表达。人源重组TGF-β3不含标签蛋白这种非糖基化蛋白分子量为12722，DTT还原蛋白为12kDa多肽，未还原的由胱氨酸连接而成的二聚体分子量为22kDa。人源重组TGF- β3的预期N-氨基末端的ALDTN由氨基酸测序验证。6 μg还原和未还原的人源重组TGF-β3由SDS-PAGE测定纯度大于98%，在非还原条件下少于5%游离物hTGF-β3以单体形式存在。所有批次的产品纯度均大于98%。重组人源TGF-β3 EGF的生物活性通过评估对IL-4诱导的HT-2细胞增殖的抑制作用测定。其每个批次的再ED50在 0.02-0.10 ng/ml之间。每1 μg hTGF-β3中内毒素含量低于0.01ng。每1 μg hTGF-β3溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干带有载体。经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物无载体。TGF-β3能诱导细胞增殖和血管生成并促进免疫反应，但它抑制其他成员[1-3]。TGF-β3由软骨胶质细胞、星状细胞核一些肿瘤细胞[3，4] TGF-β3结合TβRII二聚体然后和TβRI二聚体形成复合物[1,5]。这种低聚物受体复合物磷酸化SMAD蛋白小分子集团后再诱导或抑制某些靶标基因[1,5]。在小鼠的TGF-β3基因敲除实验中，TGF-β3对于肺和上颚发育中起关键作用[6]。

原厂资料：

Source / Purification

Recombinant human TGF-β3 (hTGF-β3) Ala301-Ser412 (Accession #NP_003230) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hTGF-β3 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,722. DTT-reduced protein migrates as a 12 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 22 kDa protein. The expected amino-terminal ALDTN of recombinant hTGF-β3 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-β3. Less than 5% migrates as monomer hTGF-β3 under non-reduced (-) conditions. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hTGF-β3 was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 0.02-0.1 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hTGF-β3.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hTGF-β3. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, pH 3.0 containing 100 mM NaCl.

Background

TGF-β3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others (1-3). TGF-β3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells (3,4). TGF-β3 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,5). The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes (1,5). Based on knock-out studies in mice, TGF-β3 is critical for lung and palate development (6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.