YAP/TAZ (D24E4) Rabbit mAb

• 产品编号：CST-8418S      品牌：CST       原厂货号：8418S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

YAP/TAZ (D24E4)Rabbit mAb兔单抗识别内源性的总YAP和总TAZ蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源TAZ蛋白的Asp362氨基酸残基序列一致。YAP蛋白(Yes相关蛋白，YAP65)因其与Yes的SH3结构域结合而被鉴定，它也会结合含有SH3结构域的其他蛋白，如Nck, Crk, Src, 和Abl（1）。除了SH3结合基序，YAP 还含有一个PDZ相互作用基序、卷曲螺旋结构域和WW结构域（2-4）。YAP的早期研究都指出它在锚定和靶定特异细胞组合时起作用，随后还发现YAP是转录共激活因子，其机理主要是是通过YAP的WW结构域与PEBP2转录因子和其他转录因子的PY基序(PPxY)相互作用（5,6）。由于在丝氨酸127的磷酸化，YAP蛋白依赖于AKT的与14-3-3蛋白相互作用，进而抑制p73介导的细胞凋亡（6）。YAP可能起转录抑制因子作用[7]，现在被认为是一种关键的Hippo (Mst1/Mst2)信号通路调控因子[8]。YAP因此在器官大小的决定和肿瘤抑制上起关键作用[8]，TAZ在Hippo信号通路中与转录共激活因子密切相关[9]。 TAZ含有一个PDZ结合基序，它与YAP的WW结构域有同源性，也被认为通过与转录因子Runx2 和 PPARγ相互作用调控间充质细胞增殖和分化间的转换，这一过程对正常组织发育和肿瘤抑制其关键作用[10]。由于它有决定MSC命运的作用，TAZ与因MSC细胞分化失调导致的一些人类疾病有临床相关性[11]。

1. Sudol, M. (1994) Oncogene 9, 2145-52.
2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
9. Yu, F.X. et al. (2012) Cell 150, 780-91.
10. Zaidi, S.K. et al. (2004) EMBO J 23, 790-9.
11. Pan, D. (2010) Dev Cell 19, 491-505.
12. Kanai, F. et al. (2000) EMBO J 19, 6778-91.
13. Hong, J.H. et al. (2005) Science 309, 1074-8.
14. Hong, J.H. and Yaffe, M.B. (2006) Cell Cycle 5, 176-9.

原厂资料：

Specificity / Sensitivity

YAP/TAZ (D24E4) Rabbit mAb recognizes endogenous levels of total YAP and TAZ proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp362 of human TAZ protein.

Background

YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6).YAP may also function as a transcriptional repressor (7) and is now recognized as a critical regulator of the Hippo (Mst1/Mst2) signaling pathway (8). YAP may therefore play an important role in both organ size determination and tumor suppression (8). 
 TAZ is a closely related transcriptional co-activator also implicated in the Hippo signaling pathway (9). Taz contains a PDZ-binding motif, which shares homology with the WW domain of YAP, and has been proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSCs) via interaction with the transcription factors Runx2 and PPARγ. This process is critical for normal tissue development and tumor suppression (10). Due to its role in the determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (11).

1. Sudol, M. (1994) Oncogene 9, 2145-52.
2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
9. Yu, F.X. et al. (2012) Cell 150, 780-91.
10. Zaidi, S.K. et al. (2004) EMBO J 23, 790-9.
11. Pan, D. (2010) Dev Cell 19, 491-505.
12. Kanai, F. et al. (2000) EMBO J 19, 6778-91.
13. Hong, J.H. et al. (2005) Science 309, 1074-8.
14. Hong, J.H. and Yaffe, M.B. (2006) Cell Cycle 5, 176-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.