CD44 (156-3C11) Mouse mAb (Pacific Blue? Conjugate)

• 产品编号：CST-8383S      品牌：CST       原厂货号：8383S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

CD44 (156-3C11) Mouse mAb (Pacific Blue™ 偶联)鼠单抗能够检测内源CD44总蛋白水平。此单克隆抗体是通过刺激人粒性白细胞来免疫BALB/c 小鼠而获得。CST公司生产的此抗体与生物素在优化条件下偶联。生物素偶联的抗体与未偶联的抗体CD44 (156-3C11) Mouse mAb #3570相同，都能够与同物种交叉反应。CD44 是I类跨膜糖蛋白，能够通过对透明质酸(HA)的亲和性或细胞外的间质(ECM)的亲和而介导细胞-细胞和细胞-间质相互作用。CD44 具有多种形态，存在多种剪切形式，并且具有多样的转录后修饰(1,2)。细胞表面CD44表达的增加是T细胞激活的特征之一，并且此蛋白在炎症应答过程中表达上调。CD44与HER2 的相互作用与卵巢癌细胞的生长有关(1-3)。CD44 能够与埃兹蛋白, 根蛋白和膜突蛋白(ERM)相互作用，这与细胞骨架连接到细胞质膜和ECM有关(4-6)。在静息细胞中，CD44的 Ser325位点被磷酸化。 PKC的激活导致Ser291位点磷酸化及Ser325位点去磷酸化，从而使得埃兹蛋白和CD44蛋白分离并发生定向运动(4)。

1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
2. Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

原厂资料：

Specificity / Sensitivity

CD44 (156-3C11) Mouse mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of total CD44 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated CD44 (156-3C11) Mouse mAb #3570.

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).

1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
2. Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze