Hedgehog Signaling Antibody Sampler Kit

• 产品编号：CST-8358S      品牌：Cell Signaling Technology       原厂货号：8358S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Shh (C9C5)兔源单克隆蛋白识别内源性的总Shh 蛋白。此抗体不与转染的IHH和DHH交叉反应。PTCH1 (C53A3)兔源单克隆抗体识别转染水平的PTCH1蛋白。此抗体也通过它免疫沉淀后的Western blot 分析检测内源性PTCH1的水平。PTCH2(G1191)抗体识别转染水平的PTCH2蛋白。它不能识别转染的PTCH1蛋白的人源PTCH1蛋白。SUFU (C54G2)兔源单克隆抗体识别内源性的总SUFU蛋白。GLI1 (V812)抗体识别内源性的总GLI1 蛋白。多克隆抗体是分别通过一种合成的肽段去免疫动物产生。这种合成的肽段分别与邻近人源PTCH2蛋白的Gly1191氨基酸残基序列一致，或者与人源GLI11蛋白邻近812位缬氨酸氨基酸残基序列一直。多克隆抗体由蛋白A和肽段亲和层析技术纯化得到。单克隆抗体是分别通过一种合成的肽段去免疫动物产生。这种合成的肽段分别与邻近人源Shh蛋白53位甘氨酸、PTCH1的1307位脯氨酸、人源SUFU的458位亮氨酸氨基酸残基序列一致。此抗体样品试剂盒提供经济节省方式来检测Hedgehog信号通路蛋白。.这种试剂盒包含足够做四次小的western blot实验所需的一抗和二抗。进化保守的Hedgehog (Hh)信号通路在胚胎发育和成人组织稳态中调节模式发育、生长和细胞迁移。Hh信号活性的异常可能与大量的出生缺陷有关，失控的Hh通路的激活与一些癌症的发展有联系[1-2]。已经确定三种脊椎动物Hh基因：Sonic (Shh), Indian (Ihh), and Desert (Dhh)三种基因既有各自独特的作用也有相同的作用[3-5]。Patched1 和Patched 2 (PTCH1 ue PTCH2)是十二个通路跨膜蛋白，也是Hh的受体[6-9]。Hh通路由一系列抑制性的相互作用组成，Hh蛋白不在时，PTCH在其他方面激活信号通路受体Smoothened (Smo)[1,2]。在此关闭状态下SUFU (Fused的抑制因子)最初是在果蝇属中作为Fused（Fu）激酶的抑制因子，通过调控转录因子Gli和Ci的定位来抑制Hh信号通路[11,12]。在果蝇中SUFU可能正向调控Hh信号，而Hh的信号依赖于SUFU蛋白水平和Hh信号强度[13] 

原厂资料：

Specificity / Sensitivity

Shh (C9C5) Rabbit mAb detects endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH. PTCH1 (C53A3) Rabbit mAb detects transfected levels of PTCH1. This antibody can also detect endogenous levels of PTCH1 through immunoprecipitation followed by Western blot analysis. PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1 protein. SUFU (C54G2) Rabbit mAb detects endogenous levels of total SUFU protein. GLI1 (V812) Antibody detects endogenous levels of total GLI1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region (predicted to be intracellular) surrounding Gly1191 of human PTCH2 or to residues surrounding Val812 of human GLI1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu53 of human Shh, Pro1307 of human PTCH1, or Leu458 of human SUFU.

Description

This sampler kit provides an economical means of evaluating key members of the Hedgehog signaling pathway. The kit contains enough primary and secondary antibody to perform four western miniblot experiments.

Background

The evolutionarily conserved Hedgehog (Hh) signaling pathway plays critical roles in the regulation of patterning, growth, and cell migration during embryonic development and adult tissue homeostasis. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh), and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the Hh receptors (6-9). The general organization of the Hh pathway consists of a series of repressive interactions. In the absence of Hh proteins (off-state), PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) (1,2). In the off-state, SUFU (Suppressor of Fused), originally identified in Drosophila as a suppressor of the Fused (Fu) kinase (10), suppresses Hh signaling by regulating the localization of the transcription factors Gli and Ci (11,12). In Drosophila, SUFU may also positively regulate Hh signaling depending on SUFU protein levels and Hh signal intensity (13).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies