Phospho-TSC2 Antibody Sampler Kit

• 产品编号：CST-8350S      品牌：Cell Signaling Technology       原厂货号：8350S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Phospho-TSC2 Antibody Sampler Kit中的每一个抗体可以识别其特异的靶目标，不会与其他家族成员发生交叉反应，除非已经说明。Phospho-Tuberin/TSC2 (Ser1387) Antibody可以检测Ser1387磷酸化的内源性Tuberin水平。本抗体可以与一个未知的140KD的蛋白发生交叉反应。单克隆抗体是采用和人类Tuberin蛋白羧基末端相对应的合成肽段或者与人类Tuberin蛋白（Thr1462）周围相对应的磷酸肽免疫动物生产的。多克隆抗体是采用与人类Tuberin蛋白 Ser939或Ser1387周围相对应的磷酸肽免疫动物生产的。多克隆抗体采用蛋白A和肽亲和层析法纯化。The Phospho-TSC2 Antibody Sampler Kit 为研究细胞内蛋白的折叠提供了经济的手段。本试剂盒包含足够的一抗和二抗来进行4次western blot实验。

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.

原厂资料：

Specificity / Sensitivity

Each antibody in the Phospho-TSC2 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Tuberin/TSC2 (Ser1387) Antibody detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody also cross-reacts with an unidentified 140 kDa protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human tuberin protein or a synthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin protein. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser939 or corresponding to residues surrounding Ser1387 of human tuberin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Phospho-TSC2 Antibody Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody.

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.