NuRD Complex Antibody Sampler Kit

• 产品编号：CST-8349S      品牌：Cell Signaling Technology       原厂货号：8349S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

CHD3 Antibody、CHD4 Antibody和MTA1 (D40D1) XP® Rabbit mAb可检测内源性的各自的总蛋白水平。MBD3 Antibody可检测内源性MBD3蛋白三个亚型(MBD3A、MBD3B、MBD3C)，以及不能与MBD2发生交叉反应。RBAP46 (V415) Antibody可识别内源性RBAP46总蛋白，并且不能与RBAP48蛋白发生交叉反应。HDAC1 (10E2) Mouse mAb和HDAC2 (3F3) Mouse mAb可检测内源性的各自总蛋白，并且不能与其它HDAC蛋白发生交叉反应。通过人工合成人源HDAC2蛋白羧基端、人源HDAC1蛋白羧基端或人源MTA1蛋白的羧基端相应的多肽片段去免疫动物从而制备出单克隆抗体。通过人工合成人源MBD3蛋白、人源CHD4蛋白、人源CHD3蛋白或人源RBAP46蛋白Val415位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化多克隆抗体。NuRD Complex Antibody Sampler Kit提供一个经济的方法去检测每个靶蛋白，这些蛋白构成了核小体重塑和去乙酰复合物(NuRD)。该试剂盒包含的一抗可进行四次免疫印迹反应。转录抑制因子NuRD (nucleosome remodeling and histone deacetylase)复合物是有多种亚单位构成，这包括HD3 (也称为Mi2-α)、CHD4 (也称为Mi2-β)、HDAC1、HDAC2、RBAP48、RBAP46、MTA1、MTA2、MTA3和MBD3 (1-6)。CHD3和CHD4都包含两个PHD(plant homeodomain)锌指结构域，该结构域能直接结合HDAC1和HDAC2。HDAC1和HDAC2具有很高的同源性，它们都涉及到组蛋白去乙酰化、染色质重塑和转录抑制，并且也被一起发现在mSin3A和MeCP1复合物(7-9)。RBAP46和RBAP48蛋白结合到组蛋白histone H4折叠区域，并且被认为可使NuRD复合物接触到组蛋白底物(10)。methyl-CpG-binding区域蛋白家族的成员MBD3和新陈代谢相关的基因MTA1、MTA2和MTA3都被发现在NuRD复合物的部分组件(2,3,7,11)。

原厂资料：

Specificity / Sensitivity

CHD3 Antibody, CHD4 Antibody, and MTA1 (D40D1) XP® Rabbit mAb recognize endogenous levels of respective total proteins. MBD3 Antibody recognizes endogenous levels of all three isoforms of the MBD3 protein (MBD3A, MBD3B, MBD3C) and does not cross-react with MBD2. RBAP46 (V415) Antibody recognizes endogenous levels of total RBAP46 protein and does not cross-react with the RBAP48 protein. HDAC1 (10E2) Mouse mAb and HDAC2 (3F3) Mouse mAb recognize endogenous levels of respective total proteins and do not cross-react with other HDAC proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the carboxy terminus of human HDAC2 protein, the carboxy terminus of human HDAC1 protein, or residues near the carboxy terminus of human MTA1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MBD3 protein, human CHD4 protein, human CHD3 protein, or residues surrounding Val415 of human RBAP46 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

NuRD Complex Antibody Sampler Kit offers an economical means of detecting each target protein that composes the nucleosome remodeling and deacetylation complex (NuRD). The kit contains enough primary antibody to perform four western blot experiments with each primary antibody.

Background

The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.