HER/ErbB Family Antibody Sampler Kit

• 产品编号：CST-8339S      品牌：Cell Signaling Technology       原厂货号：8339S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

HER/ErbB Family Antibody Sampler Kit中的每种抗体仅识别其特定靶点，且不和HER/ErbB家族的其它成员发生交叉反应。单克隆抗体是用以下蛋白或多肽免疫动物得到的：包含hEGFR的羧基端序列的融合蛋白，根据人 ErbB2 蛋白的氨基端序列合成的多肽，人ErbB3蛋白的Tyr867附近序列或人ErbB4蛋白的羧基端序列。激活态的单克隆抗体是用根据以下位点附近的序列合成磷酸化多肽免疫动物得到的：人EGFR 的Tyr1068，人ErbB2的tyrosines 1221/1222 ，人ErbB3的Tyr1222, 或人ErbB4的Tyr1284 。HER/ErbB Family Antibody Sampler Kit为检测包括EGFR, HER2/ErbB2, HER3/ErbB3和HER4/ErbB4在内的HER/ErbB家族的蛋白提供了经济有效地手段。本试剂盒还包括家族中各个蛋白相应的对照抗体。本试剂盒的每种一抗足够四次免疫印记检测实验使用。原癌基因ErbB2 (HER2)编码185kDa的受体样跨膜糖蛋白，该蛋白具有酪氨酸激酶的活性 (4)。ErbB2的配体目前尚不清楚，但在过表达的ErbB2与其它ErbB家族成员形成异源二聚体时，即使缺少配体，其酶活性仍能被激活(5)。将近40%的人类乳腺癌中能够检测到ErbB2基因的扩增及其产物的过量表达（6）。ErbB2的主要自磷酸化位点是Tyr1248和 Tyr1221/1222，这些位点的磷酸化将ErbB2与Ras-Raf-MAP激酶的信号转导途径联系起来(4,7)。HER3/ErbB3是ErbB酪氨酸激酶受体蛋白家族的成员之一，但却没有酪氨酸激酶活性。ErbB3的酪氨酸位点磷酸化依赖于其它ErbB酪氨酸激酶。与配体结合后，ErbB3与其它ErbB蛋白的异二聚体,ErbB3能够被激活的ErbB激酶磷酸化酪氨酸残基（8，9）。在ErbB3的羧基端至少有9个潜在的酪氨酸磷酸化位点。这些位点都是信号转导蛋白的结合位点，这些蛋白包括Src家族的分子，Grb2, 和PI3K激酶的p85亚基，它们都能接到ErbB的下游信号(10)。ErbB3 的Tyr1222和Tyr1289残基都位于一个YXXM基序上并参与PI3K激酶的信号传递(11)。HER4/ErbB4和家族其它成员一样，有四个胞外功能区，一个跨膜区和一个具有激活酪氨酸激酶活性的胞内区（12）。通过结合神经调节素(NRGs)和/或EGF家族配体，ErbB4形成同源二聚体或与ErbB家族的其它成员形成异二聚体，进而使受体活化进行信号传递（12）。ErbB4普遍高表达于脑和心脏。ErbB4在乳腺癌、小儿脑癌和其它种类肿瘤上的表达和正常组织发育以及肿瘤的发生均有关系（12）。

原厂资料：

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a fusion protein containing the cytoplasmic domain of human EGF receptor, a synthetic peptide corresponding to residues near the amino terminus of human ErbB2 protein, Tyr867 of human ErbB3 protein, or the carboxy-terminus of human ErbB4 protein. Activation state monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor protein, tyrosines 1221/1222 of human ErbB2 protein, Tyr1222 of human ErbB3 protein, or Tyr1284 of human ErbB4 protein.

Description

The HER/ErbB Family Antibody Sampler Kit provides an economical means to evaluate the HER/ErbB Family, including the phosphorylation of EGFR, HER2/ErbB2, HER3/ErbB3, and HER4/ErbB4. The control antibodies to each family member are also included. The kit contains enough antibody to perform four western blot experiments with each primary antibody.

Background

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (3).The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (4). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (5). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (6). The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (4,7).HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (8,9). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, GRB2, and the p85 subunit of PI3 kinase, which mediate ErbB-downstream signaling (10). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3 kinase (11).HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain, and a cytoplasmic tail containing the active tyrosine kinase domain (12). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (12). ErbB4 is ubiquitously expressed with the highest expression occurring in the brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer, and other types of carcinomas has been reported, suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (12).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.