Fatty Acid and Lipid Metabolism Antibody Sampler Kit

产品编号：CST-8335S 品牌：Cell Signaling Technology 原厂货号：8335S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

-20°C

到货周期：

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其它组分：

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb 【子货号：#11818，包装：40 µl，，运保温度：-20°C】

Fatty Acid Synthase (C20G5) Rabbit mAb 【子货号：#3180，包装：40 µl，，运保温度：-20°C】

AceCS1 (D19C6) Rabbit mAb 【子货号：#3658，包装：40 µl，，运保温度：-20°C】

Acetyl-CoA Carboxylase (C83B10) Rabbit mAb 【子货号：#3676，包装：40 µl，，运保温度：-20°C】

Phospho-ATP-Citrate Lyase (Ser455) Antibody 【子货号：#4331，包装：40 µl，，运保温度：-20°C】

ATP-Citrate Lyase Antibody 【子货号：#4332，包装：40 µl，，运保温度：-20°C】

Lipin 1 Antibody 【子货号：#5195，包装：40 µl，，运保温度：-20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度：-20°C】

ACSL1 (D2H5) Rabbit mAb 【子货号：#9189，包装：40 µl，，运保温度：-20°C】

描述：

AceCS1 (D19C6) Rabbit mAb兔单抗用于检测内源性胞质酰基辅酶A合成酶总蛋白水平。Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody用于检测在Ser79位点磷酸化的内源性ACC, 该抗体同时识别ACCα和ACCβ。Acetyl-CoA Carboxylase (C83B10) Rabbit mAb 用于检测内源性酰基辅酶A合成酶所有同工酶。Phospho-ATP-Citrate Lyase (Ser455) Antibody用于检测在Ser455位点磷酸化的内源性柠檬酸裂解酶。ACSL1 Antibody, ATP-Citrate Lyase Antibody, Fatty Acid Synthase (C20G5) Rabbit mAb, 和Lipin 1 Antibody分别用检测它们各自对应的靶蛋白。单抗的制备：用与“人源胞质酰基辅酶A合成酶序列，人源酰基辅酶A合成酶α1蛋白Ser523周围残基序列，人源脂肪酸合酶Gly46周围残基序列”相同序列的合成肽免疫动物制备而得。修饰位点特异性多抗的制备：用与“大鼠ACC蛋白Ser79周围残基序列，人源ATP柠檬酸裂解酶Ser455周围残基序列”相同序列的合成磷酸肽免疫动物制备而得。多抗的制备：用与“人源ACSL1蛋白序列，人源ATP柠檬酸裂解酶序列，人源Lipin 1蛋白Gln883周围残基序列”相同的合成肽免疫动物制备而得。抗体的纯化采用蛋白A与肽段亲和层析相结合的方法。Fatty Acid and Lipid Metabolism Antibody Sampler Kit为研究脂肪酸和脂质代谢过程中的关键蛋白水平，提供了一套经济有效的解决方案。试剂盒中的一抗至少可以满足四次Western miniblot实验。

原厂资料：

Specificity / Sensitivity

AceCS1 (D19C6) Rabbit mAb recognizes endogenous levels of total cytoplasmic acetyl-CoA synthetase. Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody recognizes endogenous levels of ACC only when phosphorylated at Ser79; this antibody recognizes both ACCα and ACCβ. Acetyl-CoA Carboxylase (C83B10) Rabbit mAb recognizes endogenous levels of all isoforms of acetyl-CoA carboxylase protein. Phospho-ATP-Citrate Lyase (Ser455) Antibody recognizes endogenous levels of ATP-citrate lyase only when phosphorylated at Ser455. ACSL1 Antibody, ATP-Citrate Lyase Antibody, Fatty Acid Synthase (C20G5) Rabbit mAb, and Lipin 1 Antibody recognize endogenous levels of their respective target proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human cytoplasmic acetyl-CoA synthetase, residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or residues surrounding Gly46 of human fatty acid synthase. Modification state-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC protein or residues surrounding Ser455 of human ATP-citrate lyase protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide derived from a sequence of human ACSL1 protein, corresponding to residues of human ATP-citrate lyase protein, or corresponding to residues surrounding Gln883 of human Lipin 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Fatty Acid and Lipid Metabolism Antibody Sampler Kit provides an economical means to evaluate key proteins involved in fatty acid and lipid metabolism. This kit includes enough primary antibody to perform four western miniblot experiments with each primary antibody.

Background

The processes of fatty acid and lipid metabolism are vital for cellular nutrient and energy maintenance. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1,2). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (4). ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for glucogenesis (5). Phosphorylation of ACL at Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (6). Fatty acid synthase (FAS) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA (7). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (8-10). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in the liver and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (11).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at -20°C. Do not aliquot the antibodies.