Lipolysis Activation Antibody Sampler Kit

产品编号：CST-8334S 品牌：Cell Signaling Technology 原厂货号：8334S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C.

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其它组分：

HSL Antibody 【子货号：#4107，包装：40 µl，，运保温度：–20°C.】

Phospho-HSL (Ser660) Antibody【子货号： #4126，包装：40 µl，，运保温度：–20°C.】

Phospho-HSL (Ser565) Antibody 【子货号：#4137，包装：40 µl，，运保温度：–20°C.】

Phospho-HSL (Ser563) Antibody 【子货号：#4139，包装：40 µl，，运保温度：–20°C.】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：40 µl，，运保温度：–20°C.】

Perilipin (D1D8) XP® Rabbit mAb 【子货号：#9349，包装：40 µl，，运保温度：–20°C.】

描述：

Lipolysis Activation Antibody Sampler Kit中的每种抗体都用于检测相应内源性的靶蛋白水平。Phospho-HSL (Ser563) Antibody不与Ser565磷酸化的HSL交叉反应。 Phospho-HSL (Ser565) Antibody不与Ser563磷酸化的HSL交叉反应。Phospho-HSL (Ser660) Antibody不与Ser563, Ser565, 和Ser659位点磷酸化的HSL交叉反应。多抗通过用与人源HSL蛋白Ser552位点(相当于大鼠HSL的Ser563), 人源HSL蛋白Ser554位点(相当于大鼠HSL的Ser565), 小鼠源HSL蛋白Ser651位点(相当于大鼠HSL的Ser660)周围残基序列相同的或人源全长的HSL的合成肽免疫动物制备而得。抗体的纯化采用蛋白A与肽段亲和层析相结合的方法。单抗通过用与人源perilipin蛋白Ile419位点周围残基序列相同的合成肽免疫动物制备而得。Lipolysis Activation Antibody Sampler Kit为检测脂肪分解过程中的多种组分的活化状态提供了一套经济有效的解决方案，其中包括磷酸化的HSL和perilipin的检测。每个试剂盒中都提供至少能够满足四次Western mini-blot实验所需的一抗。

原厂资料：

Specificity / Sensitivity

Each antibody in the Lipolysis Activation Antibody Sampler Kit detects endogenous levels of the respective target protein. Phospho-HSL (Ser563) Antibody does not cross-react with Ser565 phosphorylated HSL. Phospho-HSL (Ser565) Antibody does not cross-react with Ser563 phosphorylated HSL. Phospho-HSL (Ser660) Antibody does not cross-react with Ser563, Ser565, or Ser659 phosphorylated HSL.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser552 of human HSL (equivalent to Ser563 of rat HSL), Ser554 of human HSL (equivalent to Ser565 of rat HSL), Ser651 of mouse HSL (equivalent to Ser660 of rat HSL), or the sequence of human HSL. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin protein.

Description

The Lipolysis Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of multiple members of the lipolysis pathway, including phosphorylated HSL and perilipin. The kit includes enough antibody to perform four western mini-blot experiments with each primary antibody.

Background

Triacylglycerol is stored in lipid droplets as a primary energy reserve. During lipolysis, triacylglycerols in adipocytes are hydrolyzed into free fatty acids and glycerol. Perilipin, localized at the periphery of lipid droplets, serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin and hormone-sensitive lipase (HSL) (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as HSL (2). Phosphorylation of HSL at Ser563, Ser659, and Ser660 by PKA stimulates HSL activity, which in turn catalyzes the hydrolysis of triacylglycerol (6,7).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.