PTPN18 (D7B9) Rabbit mAb

• 产品编号：CST-8311S      品牌：CST       原厂货号：8311S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PTPN18 (D7B9)兔源单抗识别内源性的总PTPN18蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源PTPN18蛋白的Ala401氨基酸残基序列一致。PTPN18是非受体蛋白酪氨酸磷酸酶PEST家族成员之一。PEST 家族还包括PTP-PEST和PEP[1-3].。这个蛋白家族成员包括N-末端催化结构域和C端非催化结构域以及一个调节蛋白相互作用的PEST基序[4]。PTPN18最早在造血干细胞(HSCs)中被发现，并被认为对HSC分化起重要作用[1-3]。后来PTPN18显示能和c-Src酶结合形成蛋白复合体抑制Src家族酶的信号转导[4,5]。 最近还发现PTPN18在乳腺癌细胞系中的表达和HER2受体表达正相关[6,7],暗示其抑制HER2信号转导[7]。

1. Cheng, J. et al. (1996) Blood 88, 1156-67.
2. Huang, K. et al. (1996) Oncogene 13, 1567-73.
3. Dosil, M. et al. (1996) Blood 88, 4510-25.
4. Wang, B. et al. (2001) Mol Cell Biol 21, 1077-88.
5. Rubbi, L. et al. (2011) Sci Signal 4, ra18.
6. Lucci, M.A. et al. (2010) Cell Oncol 32, 361-72.
7. Gensler, M. et al. (2004) J Biol Chem 279, 12110-6.

原厂资料：

Specificity / Sensitivity

PTPN18 (D7B9) Rabbit mAb recognizes endogenous levels of total PTPN18 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala401 of human PTPN18 protein.

Background

PTPN18 is a member of the PEST family of nonreceptor protein tyrosine phosphatases, a group that also includes PTP-PEST and PEP (1-3). Members of this protein family contain an N-terminal catalytic domain and a noncatalytic C-terminal domain with a PEST motif that mediates protein-protein interactions (4). PTPN18 was first identified in hematopoietic stem cells (HSCs), where it was suggested to play a role in HSC differentiation (1-3). Subsequently, PTPN18 was shown to bind to c-Src kinase, forming a protein complex that functions to inhibit Src-family kinase signaling (4,5). More recently, PTPN18 expression has been shown to correlate with HER2 receptor expression in breast cancer cell lines (6,7), where it has been suggested to repress HER2 signaling (7).

1. Cheng, J. et al. (1996) Blood 88, 1156-67.
2. Huang, K. et al. (1996) Oncogene 13, 1567-73.
3. Dosil, M. et al. (1996) Blood 88, 4510-25.
4. Wang, B. et al. (2001) Mol Cell Biol 21, 1077-88.
5. Rubbi, L. et al. (2011) Sci Signal 4, ra18.
6. Lucci, M.A. et al. (2010) Cell Oncol 32, 361-72.
7. Gensler, M. et al. (2004) J Biol Chem 279, 12110-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody