FKBP5 Antibody

• 产品编号：CST-8245S      品牌：CST       原厂货号：8245S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FKBP5 Antibody可以识别内源性总FKBP5 蛋白。此抗体不与FKBP4发生交叉反应。多克隆抗体采用人FKBP5蛋白羧基末端残基相对应的合成肽段免疫动物生产的。抗体采用蛋白A和肽亲和层析法纯化。FK506结合蛋白（ FKBP51 ，也叫FKBP5 ）属于亲免疫蛋白FKBP家族（1）。 FKBP家族蛋白包含FK结构域和TRP（tetratricopeptide repeat）结构域。 FK结构域具有PPlase（肽基脯氨酰异构酶）的活性以及结合FK506和雷帕霉素的能力（2,3）。 FKBP5 的C端TRP结构域介导蛋白相互作用。FKBP5 的C端TRP结构域可以结合HSP90复合体（4），糖皮质激素，雄激素和黄体酮受体，从而调节类固醇激素受体的功能（5）。 FKBP5也与IKKα结合，参与NF-κB信号转导（6,7）。最近， FKBP5被确定为Akt的负调控因子（8），促进Akt – PHLPP相互作用并促进Akt的去磷酸化。

原厂资料：

Specificity / Sensitivity

FKBP5 Antibody recognizes endogenous levels of total FKBP5 protein. This antibody does not cross-react with FKBP4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FKBP5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for their PPIase (peptidylprolyl isomerase) acitivity and their ability to bind FK506 and Rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The C terminal TPR domain of FKBP5 mediates binding to HSP90 complexes (4) and glucocorticoid, androgen and progesterone receptors, which underlies its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). Recently, FKBP5 was identified as a negative regulator of Akt (8); it promotes Akt - PHLPP interaction and enhances dephosphorylation of Akt.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.