NCS1 (D12D2) XP? Rabbit mAb

• 产品编号：CST-8237P      品牌：Cell Signaling Technology       原厂货号：8237P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NCS1 (D12D2) XP® Rabbit mAb 兔单抗识别内源性的总NCS1蛋白。该单克隆抗体由人类 NCS1蛋白对应的重组蛋白免疫动物而制成。根据亲和力和功能，钙结合蛋白可以分为两类：钙缓冲剂和该传感器。钙调蛋白是一个研究的比较充分的钙传感器，在突触可塑性中的作用已经被较好的了解。神经钙传感器1（NCS1）也是该传感器家族中的一员，但是它在突触可塑性中的作用尚在研究。NCS1包含多个EF手钙结合模体，和一个氨基末端的肉豆蔻酰基团（1）。NCS1有许多结合蛋白。大多数这些蛋白都是钙依赖的（如多巴胺D2受体），少数是非钙依赖的（如IP3受体）。在小鼠齿状回，NCS1促进突触可塑性与空间记忆的快速获取（3）。

1. Amici, M. et al. (2009) Biochem Soc Trans 37, 1359-63.
2. Burgoyne, R.D. (2007) Nat Rev Neurosci 8, 182-93.
3. Saab, B.J. et al. (2009) Neuron 63, 643-56.

原厂资料：

Specificity / Sensitivity

NCS1 (D12D2) XP® Rabbit mAb recognizes endogenous levels of total NCS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human NCS1 protein.

Background

According to affinity and function, calcium-binding proteins are separated into two classes: calcium buffers and calcium sensors. Calmodulin is a well-studied calcium sensor with well-established roles in synaptic plasticity. Neuronal calcium-sensor 1 (NCS1) is also a member of the calcium sensor family, however, its role in synaptic plasticity remains under investigation. NCS1 contains multiple EF-hand calcium-binding motifs and an amino-terminal myristoyl group (1). NCS1 has a large number of binding partners. Most of these protein interactions are calcium-dependent (e.g. dopamine D2 receptor), although some are calcium-independent (e.g. IP3 receptor) (2). In murine dentate gyrus, NCS1 promotes synaptic plasticity and rapid acquisition of spatial memory (3).

1. Amici, M. et al. (2009) Biochem Soc Trans 37, 1359-63.
2. Burgoyne, R.D. (2007) Nat Rev Neurosci 8, 182-93.
3. Saab, B.J. et al. (2009) Neuron 63, 643-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody