Homer1 Antibody

• 产品编号：CST-8231S      品牌：CST       原厂货号：8231S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Homer1 Antibody 兔多抗识别内源性的总Homer1蛋白。该抗体也能识别Homer1c异构体。该多克隆抗体由合成的人类Homer1蛋白Glu130位点附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。Homer1，Homer2和Homer3是骨架蛋白，由EVH蛋白结合域、螺旋域和亮氨酸拉链结构域组成。EVH结构域是蛋白质结合的模块，可以结合富含脯氨酸的G蛋白偶联受体（GPCRs）、肌醇1,4,5-三磷酸（IP3）受体（IP3Rs）、ryanodine受体、TRP通道的PPXXF，PPXF，和LPSSP模体（1-2）。螺旋域和亮氨酸拉链结构由Homers和信号蛋白复合物多聚而成。Homer1基因编码一个短的亚型（Homer1a，aa1-186）和两个长的亚型（Homer1b，aa1-354；Homer1c，aa1-366）。Homer1a缺乏和卷曲螺旋和亮氨酸拉链结构域，可对抗Homers多聚体形成，从而解散信号蛋白复合物（3）。

1. Fagni, L. et al. (2002) Sci STKE 2002, re8.
2. Yuan, J.P. et al. (2003) Cell 114, 777-89.
3. Xiao, B. et al. (2000) Curr Opin Neurobiol 10, 370-4.

原厂资料：

Specificity / Sensitivity

Homer1 Antibody detects endogenous levels of total Homer1 protein. This antibody may also detect isoform Homer1c.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu130 of human Homer1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Homer1, Homer2 and Homer3 are scaffolding proteins, composed of an EVH protein–binding domain, a coiled-coil domain and a leucine zipper domain. The EVH domain is a protein-protein binding module that binds to the proline-rich motifs PPXXF, PPXF, and LPSSP of G protein–coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels (1-2). The coiled-coil and the leucine zipper domains cause multimerization of Homers and assemble signaling proteins complexes. The Homer1 gene encodes a short isoform (Homer1a, aa 1-186) and two long isoforms (Homer1b, aa 1-354; Homer1c, aa 1-366). Homer1a lacks the coiled-coil domain and leucine zipper, antagonizing multimerization of Homers and thus disassembling signaling proteins complexes (3).

1. Fagni, L. et al. (2002) Sci STKE 2002, re8.
2. Yuan, J.P. et al. (2003) Cell 114, 777-89.
3. Xiao, B. et al. (2000) Curr Opin Neurobiol 10, 370-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.