Human Lymphotoxin-α/TNF-β/TNFSF1 (hLT-α)

• 产品编号：CST-8230SC      品牌：CST       原厂货号：8230SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TNF Family 肿瘤坏死因子
• 应用分类：

描述：

CST公司生产的人重组蛋白LT-α (hLT-α) Pro36-Leu205 (Accession #NP_000586)是从人源293细胞表达而来。重组的hLT-α蛋白在N-末端没有标签，未糖基化的蛋白分子量据推算为18,661Da。DTT-还原和未还原的蛋白作为一个24-31kDa的蛋白转移。在SDS-PAGE中稍低的迁移率是由于糖基化。重组hLT-α蛋白的N-末端PGVGL的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hLT-α通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hLT-α的生物活性是通过L-929细胞增殖来确定的。每个批次的ED50在15-150 pg/ml之间。内毒素含量：小于0.01 ng /1 μg hLT-α。

有载体: 每微克hLT-α蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）的溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hLT-α蛋白溶解在PBS（pH 7.2）的溶液中，并通过 0.22 μm 滤膜冻干。

淋巴毒素-α (LT-α), 也叫做TNF–β，是TNF超家族蛋白的一员(1)。NK 细胞, T 细胞, B细胞和淋巴组织诱导细胞表达LT-α (1)。LT-α能作为可溶性同源三聚体而分泌或者与淋巴毒素-β (LT α1β2或者LT α2β1)形成二聚体而与膜结合，此复合体能够被细胞基质中的金属蛋白酶从细胞表面剪切(1,2)。可溶性LT-α结合到TNFR1/TNFR2并通过它传递信号, 激活常规的NF-κB通路(1)。相反, LT α1β2异源二聚体结合到LTβR受体上并激活非常规NF-κB信号通路(1)。因此, LT-α和TNF-α有重叠的功能。可溶性的LT-α和LT α1β2在淋巴管中起了重要的作用(3)。LT α1β2/LTβR 是淋巴组织发育所必不可少的(1,3)。

原厂资料：

Source / Purification

Recombinant human LT-α (hLT-α) Pro36-Leu205 (Accession #NP_000586) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hLT-α contains no "tags" and the nonglycosylated protein has a calculated MW of 18,661. DTT-reduced and non-reduced protein migrate as 24-31 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal PGVGL of recombinant hLT-α was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLT-α. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hLT-α was determined in an L-929 cell viability assay. The ED50 of each lot is between 15-150 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hLT-α.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLT-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Lymphotoxin-α (LT-α), also known as TNF–β, is a member of the TNF superfamily of proteins (1). NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-α (1). LT-α can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-β (LT α1β2 or LT α2β1) which can be cleaved from the cell surface by matrix metalloproteases (1,2). Soluble LT-α binds to and signals through TNFR1/TNFR2, activating the canonical NF-κB pathway (1). In contrast, LT α1β2 heterodimers bind to the LTβR receptor and activate the noncanonical NF-κB pathway (1). As a result, LT-α and TNF-α have overlapping functions. Soluble LT-α and LT α1β2 play key roles in lymphangiogenesis (3). The LT α1β2/LTβR axis is essential for the development of lymphoid tissue (1,3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.