CACYBP (D43G11) Rabbit mAb

• 产品编号：CST-8225S      品牌：CST       原厂货号：8225S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CACYBP (D43G11) Rabbit mAb兔单克隆抗体识别内源性的总CACYBP 蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源CACYBP蛋白的Arg162氨基酸残基序列一致。钙周期蛋白结合蛋白或Siah-1相互作用蛋白(CACYBP/SIP) 是泛素连接酶复合物E3的一个组分，此复合物也含有Siah1, Skp1, and Ebi[1] 。CACYBP调控β-catenin的翻折并对胸腺细胞发育期重要作用[2]。CACYBP也和tubulin结合可能涉及细胞骨架调控[3,4]，此蛋白在神经元高表达。它的细胞内定位可能受钙离子调控[5,6]。类维生素A作用过的成神经瘤细胞SH-SY5Y 会诱导CACYBP转移到核内，可能与CACYBP的丝氨酸残基磷酸化正相关[7]。最近的研究表明CACYBP可能有磷酸酶活性[8]，它可以结合并去磷酸化Erk1/2[8,9]。

1. Matsuzawa, S.I. and Reed, J.C. (2001) Mol Cell 7, 915-26.
2. Fukushima, T. et al. (2006) Immunity 24, 29-39.
3. Schneider, G. et al. (2007) Biochim Biophys Acta 1773, 1628-36.
4. Filipek, A. et al. (2008) J Neural Transm 115, 1257-64.
5. Jastrzebska, B. et al. (2000) J Histochem Cytochem 48, 1195-202.
6. Filipek, A. et al. (2002) J Biol Chem 277, 21103-9.
7. Wu, J. et al. (2003) J Biochem Mol Biol 36, 354-8.
8. Kilanczyk, E. et al. (2011) Biochem Biophys Res Commun 404, 179-83.
9. Kilanczyk, E. et al. (2009) Biochem Biophys Res Commun 380, 54-9.

原厂资料：

Specificity / Sensitivity

CACYBP (D43G11) Rabbit mAb recognizes endogenous levels of total CACYBP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg162 of human CACYBP protein.

Background

Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca2+ (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9).

1. Matsuzawa, S.I. and Reed, J.C. (2001) Mol Cell 7, 915-26.
2. Fukushima, T. et al. (2006) Immunity 24, 29-39.
3. Schneider, G. et al. (2007) Biochim Biophys Acta 1773, 1628-36.
4. Filipek, A. et al. (2008) J Neural Transm 115, 1257-64.
5. Jastrzebska, B. et al. (2000) J Histochem Cytochem 48, 1195-202.
6. Filipek, A. et al. (2002) J Biol Chem 277, 21103-9.
7. Wu, J. et al. (2003) J Biochem Mol Biol 36, 354-8.
8. Kilanczyk, E. et al. (2011) Biochem Biophys Res Commun 404, 179-83.
9. Kilanczyk, E. et al. (2009) Biochem Biophys Res Commun 380, 54-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.