PhosphoPlus? S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

• 产品编号：CST-8207S      品牌：CST       原厂货号：8207S
• 产品分类：抗体 > 一抗 > 抗体对
• 应用分类：

其它组分：

描述：

来自Cell Signaling Technology (CST) 的PhosphoPlus® Duets提供了评价蛋白质激活状态的一种方法。每一个抗体对都含有你感兴趣蛋白的激活状态和总的蛋白抗体。它们是从CST的产品中精挑细选出来的，对于特定的应用具有绝佳的表现。生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关，而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

原厂资料：

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.