Mouse Lipocalin-2 (mLCN2)

• 产品编号：CST-8199SF      品牌：CST       原厂货号：8199SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

重组小鼠LCN2 (mLCN2) Gln21-Asn200 (Accession #P11672)由 CST公司在人293T细胞中进行表达。重组mLCN2没有标签，非糖基化的蛋白分子量为20,874Da。DTT还原的和非还原的蛋白接近24kDa多肽的迁移位置。用6 μg还原的(+)和非还原的(-)重组mLCN2进行SDS-PAGE，纯度都大于98%。所有批次的产品纯度都大于98%。用BAF3细胞生存试验进行mLCN2的生物活性测定。每个批次的ED50在0.5-4 µg/ml之间。每微克mLCN2内毒素含量小于0.01ng。

有载体：经0.22μm滤膜过滤的0.33 mg/ml mLCN2溶液（20mM Tris，pH8.0，150 mM NaCl，每1 μg mLCN2中含20 μg BSA）。

无载体：经0.22μm滤膜过滤的0.33 mg/ml mLCN2溶液（20mM Tris，pH8.0，150 mM NaCl）。

脂质运载蛋白2是脂质运载蛋白家族的成员，最初是作为嗜中性粒细胞分泌颗粒的明胶酶相关成分被发现的（1）。脂质运载蛋白2与先天免疫、铁稳态和细胞凋亡有关。脂质运载蛋白2通过结合细菌的铁载体和隔离铁离子来限制细菌生长（2-4）。在哺乳动物细胞中，装载铁离子的脂质运载蛋白2与其受体24p3R结合，并被纳入，从而释放铁离子，使细胞内铁离子浓度增加（5）。相反的，未装载铁离子的脂质运载蛋白2能促进细胞凋亡（5）。脂质运载蛋白2在脂肪组织中也能表达，能提高培养的小鼠脂细胞的胰岛素抗药性（6）。

原厂资料：

Source / Purification

Recombinant mouse LCN2 (mLCN2) Gln21-Asn200 (Accession #P11672) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mLCN2 contains no "tags" and the nonglycosylated protein has a calculated MW of 20,874. DTT-reduced and non-reduced proteins migrate as 24 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mLCN2 All lots are greater than 98% pure.

Bioactivity

The bioactivity of mLCN2 was determined in a BAF3 cell viability assay. The ED50 of each lot is between 0.5-4 µg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mLCN2.

Formulation

With carrier: A 0.22 μm filtered solution of 0.33 mg/ml mLCN2 in 20 mM Tris, pH 8.0 containing 150 mM NaCl and 20 μg BSA per 1 μg mLCN2. Carrier free: A 0.22 μm filtered solution of 0.33 mg/ml mLCN2 in 20 mM Tris, pH 8.0 containing 150 mM NaCl.

Background

Lipocalin-2, a member of the lipocalin family of proteins, was originally identified as a gelatinase-associated component of neutrophil secretory granules (1). Lipocalin-2 is involved in innate immunity, iron homeostasis, and apoptosis. Lipocalin-2 limits bacterial growth by binding to bacterial siderophores and sequestering iron (2-4). In mammalian cells, iron-loaded lipocalin-2 binds to its receptor, 24p3R, and is internalized, thereby releasing iron and increasing the intracellular iron concentration (5). Conversely, iron-free lipocalin-2 promotes apoptosis (5). Lipocalin-2 is also expressed in adipose tissue and promotes insulin resistance in cultured mouse adipocytes (6).

注意事项：

Storage: Stable at 4ºC for 1 year after receipt