PKD2 (D1A7) Rabbit mAb

• 产品编号：CST-8188S      品牌：CST       原厂货号：8188S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PKD2 (D1A7)兔单克隆抗体识别内源性的全部PKD2蛋白。此抗体与PKD1或PKD3蛋白没有交叉反应。该单克隆抗体用与人类PKD2蛋白中Gly491位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。蛋白激酶 D2 (PKD2)是蛋白激酶D家族中三个成员之一，包括PKD1/PKCμ 和PKD3/PKCν, 属于丝氨酸/苏氨酸蛋白激酶的钙/钙调蛋白超家族(1,2)。PKDs包含一个保守的羧基末端催化结构域，一个PH结构域标记的氨基末端调节区用以进行亚细胞坐标定位，两个锌指/C1脂质结合结构域用以调节响应甘油二酯（DAG）或佛波酯的酶活性（2,3）。除了脂质介导的激活，也可以通过酶活化环关键丝氨酸残基的磷酸化来刺激PKD的催化活性（4-8）。新型PKCs，如PKCη 和PKCε,已经显示出使PKD1 丝氨酸（744位和748位）(人PKD2中是丝氨酸（706和710位）)磷酸化，从而通过PH结构域与催化结构域的相互作用，介导缓和酶抑制作用(5)。PKD亚型的磷酸化和激活也用于其他上游激酶的描述。例如，酪蛋白激酶2 (CK2)已经显示出PKD2 丝氨酸（244位）磷酸化，这将促进核内PKD2的积累、HDAC7的磷酸化和Nur77的表达(9)。虽然只有少量PKD2效应物已经确定，PKD2与一系列细胞活动的调节有关，包括细胞存活、发育、生长、迁移和转化（10-14）。PKD2介导至少一个已知底物的磷酸化，磷脂酰肌醇4 - 激酶的IIIβ（PI4KIIIβ）型，也与来自反式高尔基网络形成和调节胞吐运输小泡中的PKD2有关（15）。

1. Rykx, A. et al. (2003) FEBS Lett 546, 81-6.
2. Sturany, S. et al. (2001) J Biol Chem 276, 3310-8.
3. Chen, J. et al. (2008) Biochem J 411, 333-42.
4. Zugaza, J.L. et al. (1996) EMBO J 15, 6220-30.
5. Waldron, R.T. et al. (2001) J Biol Chem 276, 32606-15.
6. Waldron, R.T. and Rozengurt, E. (2003) J Biol Chem 278, 154-63.
7. Sinnett-Smith, J. et al. (2009) J Biol Chem 284, 13434-45.
8. Konopatskaya, O. et al. (2011) Blood, Epub ahead of print.
9. von Blume, J. et al. (2007) EMBO J 26, 4619-33.
10. Mihailovic, T. et al. (2004) Cancer Res 64, 8939-44.
11. Irie, A. et al. (2006) Int Immunol 18, 1737-47.
12. Sinnett-Smith, J. et al. (2007) J Cell Physiol 211, 781-90.
13. Hao, Q. et al. (2009) J Biol Chem 284, 799-806.
14. Kleger, A. et al. (2011) PLoS One 6, e14599.
15. Pusapati, G.V. et al. (2010) Mol Biol Cell 21, 1011-22.

原厂资料：

Specificity / Sensitivity

PKD2 (D1A7) Rabbit mAb recognizes endogenous levels of total PKD2 protein. This antibody does not cross react with PKD1 or PKD3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly491 of human PKD2 protein.

Background

Protein kinase D2 (PKD2) is one of three members of the protein kinase D family, including PKD1/PKCμ and PKD3/PKCν, that belong to the calcium/calmodulin superfamily of serine/threonine protein kinases (1,2). PKDs contain a conserved, carboxy-terminal catalytic domain, an amino-terminal regulatory region hallmarked by a PH domain that coordinates subcellular localization, and two zinc-finger/C1 lipid-binding domains that mediate activation of the enzyme in response to diacylglycerol (DAG) or phorbol ester (2,3). In addition to lipid-mediated activation, PKD catalytic activity can also be stimulated via phosphorylation of critical serine residues within the activation loop of the enzyme (4-8). Novel PKCs, such as PKCη and PKCε, have been shown to phosphorylate PKD1 at Ser744 and Ser748 (Ser706 and Ser710 in human PKD2), resulting in alleviation of autoinhibition of the enzyme mediated by PH domain interactions with the catalytic domain (5). Phosphorylation and activation of PKD isoforms has also been described for other upstream kinases. For example, casein kinase 2 (CK2) has been shown to phosphorylate PKD2 at Ser244, which promotes nuclear accumulation of PKD2, phosphorylation of HDAC7, and expression of Nur77 (9). Although only a handfull of PKD2 effectors have been identified, PKD2 has been implicated in regulating an array of cellular events, including cell survival, development, growth, migration, and transformation (10-14). PKD2-mediated phosphorylation of at least one known substrate, phosphatidylinositol 4-kinase type IIIβ (PI4KIIIβ), also implicates PKD2 in the formation and regulation of exocytotic transport vesicles from the trans Golgi network (15).

1. Rykx, A. et al. (2003) FEBS Lett 546, 81-6.
2. Sturany, S. et al. (2001) J Biol Chem 276, 3310-8.
3. Chen, J. et al. (2008) Biochem J 411, 333-42.
4. Zugaza, J.L. et al. (1996) EMBO J 15, 6220-30.
5. Waldron, R.T. et al. (2001) J Biol Chem 276, 32606-15.
6. Waldron, R.T. and Rozengurt, E. (2003) J Biol Chem 278, 154-63.
7. Sinnett-Smith, J. et al. (2009) J Biol Chem 284, 13434-45.
8. Konopatskaya, O. et al. (2011) Blood, Epub ahead of print.
9. von Blume, J. et al. (2007) EMBO J 26, 4619-33.
10. Mihailovic, T. et al. (2004) Cancer Res 64, 8939-44.
11. Irie, A. et al. (2006) Int Immunol 18, 1737-47.
12. Sinnett-Smith, J. et al. (2007) J Cell Physiol 211, 781-90.
13. Hao, Q. et al. (2009) J Biol Chem 284, 799-806.
14. Kleger, A. et al. (2011) PLoS One 6, e14599.
15. Pusapati, G.V. et al. (2010) Mol Biol Cell 21, 1011-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody