Phospho-LDHA (Tyr10) Antibody

• 产品编号：CST-8176S      品牌：CST       原厂货号：8176S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-LDHA (Tyr10)抗体识别内源性的Tyr10磷酸化的LDHA蛋白水平。该多克隆抗体用与人类LDHA中Tyr10位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。乳酸脱氢酶（LDH）催化丙酮酸和NADH转化为乳酸和NAD+。当供给线粒体制造ATP的氧气太少时，这个反应将在糖酵解中循环NADH生成NAD+。LDH在肌细胞中的主要形式是A（LDHA）亚型。LDHA启动子包括HIF-1α结合域（1）。LDHA表达在低氧情况下被诱导（2）。在高强度和长时间肌肉活动中，供氧不能满足需求，乳酸在肌细胞内堆积。当供氧恢复正常时，LDH将乳酸转化为丙酮酸并在线粒体电子传递链中产生ATP。最近的研究表明LDHA被受体酪氨酸激酶FGFR在Try10和Tyr83磷酸化（3）。Tyr10的磷酸化可以上调LDHA的活性，并且在多种人类癌细胞中发现（3）。研究结果进一步推测酪氨酸磷酸化调节NADH/NAD+ redox的动态平衡，并且促进Warburg效应和肿瘤细胞生长（3）。

1. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
2. Semenza, G.L. (2007) Biochem J 405, 1-9.
3. Fan, J. et al. (2011) Mol Cell Biol 31, 4938-50.

原厂资料：

Specificity / Sensitivity

Phospho-LDHA (Tyr10) Antibody recognizes endogenous levels of LDHA protein only when phosphorylated at Tyr10.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr10 of human LDHA protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.Recent studies indicate that LDHA is phosphorylated at Tyr10 and Tyr83 by the receptor tyrosine kinase FGFR1 (3). Phosphorylation at Tyr10 up-regulates the activity of LDHA and is found in a variety of human cancer cells (3). Results further suggest that tyrosine phosphorylation regulates the NADH/NAD+ redox homeostasis and thus promotes the Warburg effect and tumor cell growth (3).

1. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
2. Semenza, G.L. (2007) Biochem J 405, 1-9.
3. Fan, J. et al. (2011) Mol Cell Biol 31, 4938-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.