PFKL Antibody

• 产品编号：CST-8175S      品牌：CST       原厂货号：8175S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PFKL抗体识别内源性的PFKL总蛋白水平。该多克隆抗体用与人类PFKL蛋白羧基末端附近的氨基酸链对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。磷酸果糖激酶(PFK)催化糖酵解中果糖-6-磷酸的磷酸化（1）。它有三种亚型：肌肉型、肝脏型和血小板型（2）。肝脏型PKF（PKFL）是肝脏中PFK的主要亚型（1,3）。研究表明因为其定位于21号染色体，该酶在唐氏综合征症21三体综合症中过度表达（3,4）。PFKL也在低脂饮食中的生理反应中发挥作用（5）。

1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
3. Elson, A. et al. (1994) Biochem J 299 ( Pt 2), 409-15.
4. Peled-Kamar, M. et al. (1998) Brain Res 810, 138-45.
5. Seip, R.L. et al. (2008) Nutr Metab (Lond) 5, 4.

原厂资料：

Specificity / Sensitivity

PFKL Antibody recognizes endogenous levels of total PFKL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PFKL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2). The liver-type PFK (PFKL) is the predominant subunit of PFK found in the liver (1,3). Research studies have shown that because of its location on chromosome 21, this enzyme is overexpressed in Down Syndrome/trisomy 21 (3,4). PFKL also may have a role in the physiological response to a low fat diet (5).

1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
3. Elson, A. et al. (1994) Biochem J 299 ( Pt 2), 409-15.
4. Peled-Kamar, M. et al. (1998) Brain Res 810, 138-45.
5. Seip, R.L. et al. (2008) Nutr Metab (Lond) 5, 4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody