Enolase-2 (D20H2) Rabbit mAb

• 产品编号：CST-8171S      品牌：CST       原厂货号：8171S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Enolase-2 (D20H2)兔单克隆抗体识别内源性的enolase-2总蛋白水平。该单克隆抗体用与人类enolase-2蛋白中羧基末端附近的氨基酸序列对应的人工合成肽段免疫动物制成。烯醇酶是一种糖酵解酶，涉及2-磷酸到磷酸烯醇式丙酮酸的转化（1）。哺乳动物烯醇酶有三个亚：α, β和γ，可以生成同源或异源二聚体。γ烯醇酶二聚体是非特异性的（2）。研究显示神经特异性烯醇酶-2在神经母细胞瘤（2）和小细胞肺癌（3,4）中表达升高。

1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

原厂资料：

Specificity / Sensitivity

Enolase-2 (D20H2) Rabbit mAb recognizes endogenous levels of total enolase-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human enolase-2 protein.

Background

Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4).

1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.