Human Interleukin-7 (hIL-7)

• 产品编号：CST-8170SC      品牌：CST       原厂货号：8170SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司利用重组的人IL-7 (hIL-7) Asp26-His177 (Accession #NP_000871)在E. coli细胞中表达。重组的hIL-7蛋白在N-末端有Met标签，其分子量据推算为17,518Da。DTT-还原和未还原的蛋白迁移时是作为一个17kDa 的多肽而转移。重组hIL-7蛋白的N-末端序列MDCDI通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-7通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hIL-7的生物活性是通过2E8细胞增殖实验确定的。每个批次的ED50在90-100pg/ml之间。内毒素含量:<0.01 ng /1μg hIL-7。

有载体: 每微克hIL-7蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-7蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-7 在淋巴细胞增殖和淋巴细胞的动态平衡中发挥了重要的作用(1)。在骨髓和胸腺中的基质和上皮细胞产生IL-7(1)。IL-7最主要的靶标是T细胞、B细胞和树突细胞(1)。IL-7对T细胞的发育非常重要，此重要性在缺失T细胞的小鼠和人中的IL-7/IL-7R信号缺陷中被低估(1,2)。同时，在小鼠中IL-7是B细胞的发育中必需的, IL-7在人的B细胞发育中的作用尚不清楚(1,3)。除了它在T淋巴细胞增殖中的影响, IL-7促进空白和记忆αβ T和γδ T细胞的维持和存活(1)。

原厂资料：

Source / Purification

Recombinant human IL-7 (hIL-7) Asp26-His177 (Accession #NP_000871) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-7 has a Met on the amino terminus and has a calculated MW of 17,518. DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides. The expected amino-terminal MDCDI of recombinant hIL-7 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-7. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 90-1000 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg hIL-7.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory αβ T cells, as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7-specific IL-7Rα (1). IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5 (1).

注意事项：

should be greater than 50 μg/ml.
Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.