PFKP (D4B2) Rabbit mAb

• 产品编号：CST-8164S      品牌：CST       原厂货号：8164S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PFKP (D4B2) Rabbit mAb 兔单抗能够检测内源性PFKP总蛋白水平。该单克隆抗体是由合成的人源PFKP蛋白N末端肽段免疫动物制备的。磷酸果糖激酶(PFK)在糖酵解过程中催化6-磷酸果糖的磷酸化（1）。它有三种同工酶：肌肉型，肝脏型和血小板型（2,3）。血小板型的磷酸果糖激酶(PFKP)在多种细胞中表达（4,5）。研究发现，幼龄时发生PFKP基因变异的个体在成年后易患肥胖和糖尿病（6）。

1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
3. Hannemann, A. et al. (2005) Gene 345, 237-47.
4. Morrison, N. et al. (1992) Hum Genet 89, 105-6.
5. Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
6. Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.

原厂资料：

Specificity / Sensitivity

PFKP (D4B2) Rabbit mAb recognizes endogenous levels of total PFKP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PFKP protein.

Background

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2,3). Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (4,5). Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood (6).

1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
3. Hannemann, A. et al. (2005) Gene 345, 237-47.
4. Morrison, N. et al. (1992) Hum Genet 89, 105-6.
5. Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
6. Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.