Oct-1 (D7B6) Rabbit mAb

• 产品编号：CST-8157S      品牌：CST       原厂货号：8157S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Oct-1 (D7B6) Rabbit mAb兔单抗识别内源性的总Oct-1蛋白。合成与人源Oct-1蛋白邻近Ala367氨基酸残基序列的肽段，免疫动物获得单克隆抗体。Oct-1 (POU2F1)是一个广泛表达，以八聚体形式发挥作用的转录因子，它带有一个内含同源序列的POU结构域（1）。Oct-1可以通过与一些转录因子结合介导特异基因表达，包括SNAPc（2），OBF-1 (a B-cell 转录辅助激活蛋白) (3), TFIIB (4), 和TBP (TATA-box结合蛋白) (5)。其带有的POU的 DNA结合结构域使Oct-1能够结合与招募一些组织特异性的辅助因子以正向或负向地调控不同的基因，在发育过程中发挥重要作用（6）。

1. Sturm, R.A. et al. (1988) Genes Dev 2, 1582-99.
2. Mittal, V. et al. (1996) Mol Cell Biol 16, 1955-65.
3. Strubin, M. et al. (1995) Cell 80, 497-506.
4. Nakshatri, H. et al. (1995) J Biol Chem 270, 19613-23.
5. Zwilling, S. et al. (1994) Nucleic Acids Res 22, 1655-62.
6. Schonemann, M.D. et al. (1998) Adv Exp Med Biol 449, 39-53.

原厂资料：

Specificity / Sensitivity

Oct-1 (D7B6) Rabbit mAb recognizes endogenous levels of total Oct-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human Oct-1 protein.

Background

Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6).

1. Sturm, R.A. et al. (1988) Genes Dev 2, 1582-99.
2. Mittal, V. et al. (1996) Mol Cell Biol 16, 1955-65.
3. Strubin, M. et al. (1995) Cell 80, 497-506.
4. Nakshatri, H. et al. (1995) J Biol Chem 270, 19613-23.
5. Zwilling, S. et al. (1994) Nucleic Acids Res 22, 1655-62.
6. Schonemann, M.D. et al. (1998) Adv Exp Med Biol 449, 39-53.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody