IDH1 (D2H1) Rabbit mAb

• 产品编号：CST-8137S      品牌：CST       原厂货号：8137S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IDH1 (D2H1)兔单克隆抗体识别内源性的IDH1总蛋白水平。此抗体不识别内源性的IDH2蛋白水平。该单克隆抗体用与人类IDH1蛋白中Arg222位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。IDH1是三种异柠檬酸脱氢酶之一，催化异柠檬酸氧化脱羧形成α-酮戊二酸（α-KG）。这些酶存在两个不同的亚型，分别利用NAD或NADP+作为电子受体（1）。IDH1是NADP+以来的异柠檬酸脱氢酶，在细胞质和过氧化物酶体中发现。IDH2和3是线粒体酶，也在Kreb循环中起作用。IDH1被在Ser113磷酸化而失活，它包含一个钩状的结构域，二聚体中的多肽链在这个部位相互锁扣（2,3）。IDH1在多个物种表达，甚至在缺乏完整柠檬酸循环的生物中也有表达。最近，IDH1的一种失活突变在神经母细胞瘤中被发现（4）。IDH1似乎有肿瘤抑制因子的作用，当突变失活后，可通过诱导HIF-1途径促进肿瘤生成（5）。

1. Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
2. Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
3. Hurley, J.H. et al. (1990) Science 249, 1012-6.
4. Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
5. Zhao, S. et al. (2009) Science 324, 261-5.

原厂资料：

Specificity / Sensitivity

IDH1 (D2H1) Rabbit mAb recognizes endogenous levels of total IDH1 protein. This antibody does not recognize endogenous IDH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg222 of human IDH1 protein.

Background

IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).

1. Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
2. Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
3. Hurley, J.H. et al. (1990) Science 249, 1012-6.
4. Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
5. Zhao, S. et al. (2009) Science 324, 261-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.