Phospho-PZR (Tyr241) (D6F9) Rabbit mAb

• 产品编号：CST-8131S      品牌：CST       原厂货号：8131S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化PZR (酪氨酸241) (D6F9)兔源单抗仅在241位酪氨酸磷酸化时识别内源性PZR 蛋白水平。单抗由合成肽段免疫动物产生，该合成肽段对应人PZR蛋白的241位酪氨酸附近残基。PZR(蛋白零点相关)是免疫球蛋白超家族蛋白，通过胞内免疫受体酪氨酸为基础的抑制性基序（ITIMs）特异结合酪氨酸激酶SHP-2（1,2）。PZR 被c-Src, c-Fyn, c-Lyn, Csk, 和 c-Abl磷酸化（3）。PP1,Src家族激酶抑制剂，抑制PZR磷酸化（4,5）。有三种选择性剪接异构体，命名为PZR, PZRa, 和 PZRb；PZRa 和 PZRb缺乏ITIMs（6,7）。PZR是主要的ConA受体，通过c-Src在细胞信号中起重要作用（4）。PZR在多种细胞中表达，定位在BAECs和间皮瘤细胞的细胞接触和胞内颗粒中。PZR 涉及细胞粘附蛋白，可能涉及SHP-2依赖的信号通路内皮细胞接触中（3）。PZR的高酪氨酸磷酸化在努南综合征的小鼠模型的胚胎发育中被观察到（8）。利用ConA处理或双氧水处理，两个PZR胞内ITIM酪氨酸位点241和263被磷酸化（4,8）。这两个位点的磷酸化帮组招募SHP-2到PZR，PZR改变SHP-2的磷酸化酶活性，影响它的下游信号（5，8，9）。

1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
3. Kusano, K. et al. Endothelium 15, 127-36.
4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.
9. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.

原厂资料：

Specificity / Sensitivity

Phospho-PZR (Tyr241) (D6F9) Rabbit mAb recognizes endogenous levels of PZR protein only when phosphorylated at Tyr241.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr241 of human PZR protein.

Background

PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).Upon Con A treatment or H2O2 treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9).

1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
3. Kusano, K. et al. Endothelium 15, 127-36.
4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.
9. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.