Rab10 (D36C4) XP? Rabbit mAb

• 产品编号：CST-8127P      品牌：Cell Signaling Technology       原厂货号：8127P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab10 (D36C4) XP® Rabbit mAb兔单抗识别内源性Rab10总蛋白。该单克隆抗体用与人类Rab10蛋白中Gly190位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。Rab10是小Rab GTP酶Ras超家族的一员（1），可以与Mss4、myosin V（Va、Vb和Vc）和GDI相互作用，其介导细胞内体的分选（2-4）。在MDCK细胞对Rab10进行突变分析和GFP融合蛋白表达发现Rab10在早期内体和基底侧膜区域蛋白转运中发挥调节作用（5,6）。Rab10结合GLUT4复合物，作为AS160的靶标，并且为脂肪细胞中胰岛素刺激的GLUT4转位所需（7,8）。

1. Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
2. Burton, J.L. et al. (1994) EMBO J 13, 5547-58.
3. Roland, J.T. et al. (2009) J Biol Chem 284, 1213-23.
4. Chen, Y. et al. (2009) Biochem J 422, 229-35.
5. Schuck, S. et al. (2007) Traffic 8, 47-60.
6. Babbey, C.M. et al. (2006) Mol Biol Cell 17, 3156-75.
7. Sano, H. et al. (2007) Cell Metab 5, 293-303.
8. Larance, M. et al. (2005) J Biol Chem 280, 37803-13.

原厂资料：

Specificity / Sensitivity

Rab10 (D36C4) XP® Rabbit mAb recognizes endogenous levels of total Rab10 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab10 protein.

Background

Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc), and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8).

1. Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
2. Burton, J.L. et al. (1994) EMBO J 13, 5547-58.
3. Roland, J.T. et al. (2009) J Biol Chem 284, 1213-23.
4. Chen, Y. et al. (2009) Biochem J 422, 229-35.
5. Schuck, S. et al. (2007) Traffic 8, 47-60.
6. Babbey, C.M. et al. (2006) Mol Biol Cell 17, 3156-75.
7. Sano, H. et al. (2007) Cell Metab 5, 293-303.
8. Larance, M. et al. (2005) J Biol Chem 280, 37803-13.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.