SignalSlide? PTEN IHC Controls

• 产品编号：CST-8106S      品牌：CST       原厂货号：8106S
• 产品分类：生化和细胞分析 > 其它细胞分析试剂盒 > IHC 和 ICC 试剂盒 > 组织切片
• 应用分类：

描述：

每一个对照玻片包含经过福尔马林固定，石蜡包埋的d LNCaP 和NIH/3T细胞团。 NIH/3T3细胞表达PTEN而LNCaP 细胞不表达PTEN。Western blot采用相同细胞提取物来验证PTEN的表达。这些玻片应用于免疫组化实验中。请在配套产品中查看适用于这些对照片的产品列表。PTEN（phosphatase and tensin homologue deleted on chromosome ten），也称为MMAC（ (mutated in multiple advanced cancers)磷酸酶，是一种存在于多种人类癌症中的肿瘤抑制因子（1）。PTEN编码一个403个氨基酸组成的多肽，最初被认为是一个双特异性蛋白磷酸酶（2）。PTEN的主要底物是3磷酸肌醇激酶（PI3K）激活转化生成的肌醇磷脂（3）。PTEN是PI3K/Akt信号通路的一个主要的负调因子（1,4,5）。PTEN有一个羧基端的非催化调控的结构域，其中含有3个可以调节PTEN的稳定性，也可能影响其生物活性的磷酸化位点（Ser380，Thr382，Thr383）（6,7）。PTEN调节p53蛋白水平和活性（8），并在趋化过程中参与G蛋白偶联信号转导（9,10）。

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

原厂资料：

Description

Each control slide contains formalin fixed, paraffin-embedded LNCaP and NIH/3T3 cell pellets. NIH/3T3 cells express PTEN while LNCaP cells do not express PTEN. Western blot analysis was performed on extracts derived from the same cells to verify PTEN expression.

Applications

These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of products that can be used with these slides.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

注意事项：

Storage: Store at 4°C.