SignalSlide? Phospho-Stat1/3/5 IHC Controls

• 产品编号：CST-8105S      品牌：Cell Signaling Technology       原厂货号：8105S
• 产品分类：生化和细胞分析 > 其它细胞分析试剂盒 > IHC 和 ICC 试剂盒 > 组织切片
• 应用分类：

描述：

每一张对照片子上有福尔马林固定，石蜡包埋的未经过处理的和经过干扰素alpha (IFN-α)处理的786-0细胞 , 作为Phospho-Stat1 (Tyr701), Phospho-Stat3 (Tyr705) 和Phospho-Stat5 (Tyr694)免疫染色的对照。通过Western Blot分析这些细胞提取液，以确定IFN-alpha处理的有效性。SignalSlide™ Phospho-Stat1/3/5 IHC Control 片子尚未经过测试作为Phospho-Stat1 (Ser727)或Phospho-Stat3 (Ser727)免疫染色的对照。Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1)，并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) ，而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活，从而导致它的二聚化，入核并与DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化的调节(8,9)。Stat3不同剪切形式的表达反应了其生物学功能，Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型、配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

1. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
2. Bromberg, J. (2002) J Clin Invest 109, 1139-42.
3. Frank, D.A. (1999) Mol Med 5, 432-56.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
6. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
7. Darnell, J.E. (1997) Science 277, 1630-5.
8. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
9. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
10. Buitenhuis, M. et al. (2004) Int J Biochem Cell Biol 36, 2120-4.
11. Baśkiewicz-Masiuk, M. et al. (2003) Cell Prolif 36, 265-78.

原厂资料：

Description

Each control slide contains formalin fixed, paraffin-embedded 786-0 cells, both untreated and treated with interferon alpha (IFN-α), that serve as a control for Phospho-Stat1 (Tyr701), Phospho-Stat3 (Tyr705) and Phospho-Stat5 (Tyr694) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the IFN-alpha treatment. SignalSlide™ Phospho-Stat1/3/5 IHC Control slides have not been tested as controls for Phospho-Stat1 (Ser727) or Phospho-Stat3 (Ser727) immunostaining.

Applications

These slides are intended for use in immunohistochemical assays. Please see our Companion Products list for products that can be used with these slides.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

1. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
2. Bromberg, J. (2002) J Clin Invest 109, 1139-42.
3. Frank, D.A. (1999) Mol Med 5, 432-56.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
6. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
7. Darnell, J.E. (1997) Science 277, 1630-5.
8. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
9. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
10. Buitenhuis, M. et al. (2004) Int J Biochem Cell Biol 36, 2120-4.
11. Baśkiewicz-Masiuk, M. et al. (2003) Cell Prolif 36, 265-78.

注意事项：

Storage: Store at 4oC.