SignalSlide? Cleaved Caspase-3 (Asp175) IHC Controls

• 产品编号：CST-8104S      品牌：CST       原厂货号：8104S
• 产品分类：生化和细胞分析 > 其它细胞分析试剂盒 > IHC 和 ICC 试剂盒 > 组织切片
• 应用分类：

描述：

每一组对照片包含福尔马林固定片和石蜡包埋切片，未处理和etoposide 处理的Jurkat细胞，它们作为剪切caspase-3 (Asp 175)免疫染色的对照组。Western blot分析从相同细胞得到的提取物，以此来验证依托 etoposide 处理的效率。Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者，因为它对许多关键的蛋白起着部分或全部的裂解作用，比如核酶多聚(ADP-ribose)聚合酶(PARP)（1）。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点（2）。

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

原厂资料：

Description

Each control slide contains formalin fixed, paraffin-embedded Jurkat cells, both untreated and treated with etoposide, that serve as a control for cleaved caspase-3 (Asp 175) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the etoposide treatment.

Applications

These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of products that can be used with these slides.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

注意事项：

Storage: Store at 4oC.