TECPR1 (D6C10) Rabbit mAb

• 产品编号：CST-8097S      品牌：CST       原厂货号：8097S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TECPR1 (D6C10) Rabbit mAb 兔单抗能够识别内源性的TECPR1总蛋白。这种抗体与TECPR2不发生反应。在某些细胞系中，检测到 200kDa 左右的未知来源的背景条带。该单克隆抗体是通过合成肽免疫动物制备的，这种合成肽与人源的TECPR1蛋白His900周围的残基相一致。Tectonin β-propeller repeat protein (TECPR1)首次被鉴定为DKFZP434B0335，一种酵母蛋白Pex23p的人类同源物(1)。TECPR1含有一个PH(pleckstrin homology)结构域、β-螺旋域和 dysferlin域。研究表明，TECPR1表达量的提高可以作为前列腺癌的一个潜在标志物(2)。在几个独立的研究中，TECPR1能够与Atg5相互作用，从而在自噬中发挥一定的作用(3-5)。Atg5是一种能够与泛素样蛋白Atg12相结合的蛋白，在自噬中起着至关重要的作用(6)。初步研究表明，TECPR1在选择性自噬过程中，通过靶向细菌病原体而发挥一定的作用，如受损的线粒体和蛋白质聚集物(4)。TECPR1对于自噬小体的成熟，以及促进自噬小体与溶酶体融合方面起着重要的作用(5)。缺失TECPR1会导致自噬小体的累积(5)。

1. Jeynov, B. et al. (2006) FEBS Lett 580, 5917-24.
2. Bull, J.H. et al. (2001) Br J Cancer 84, 1512-9.
3. Behrends, C. et al. (2010) Nature 466, 68-76.
4. Ogawa, M. et al. (2011) Cell Host Microbe 9, 376-89.
5. Chen, D. et al. (2012) Mol Cell 45, 629-41.
6. Mizushima, N. et al. (1998) Nature 395, 395-8.

原厂资料：

Specificity / Sensitivity

TECPR1 (D6C10) Rabbit mAb recognizes endogenous levels of total TECPR1 protein. This antibody does not cross-react with TECPR2. A background band of unknown origin around 200 kDa is detected in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His900 of human TECPR1 protein.

Background

Tectonin β-propeller repeat protein (TECPR1) was first identified as DKFZP434B0335, a human ortholog of the yeast protein Pex23p (1). TECPR1 contains a pleckstrin homology (PH) domain, β-propeller domain, and dysferlin domains. Research studies have shown that elevated expression of TECPR1 may be a potential marker for prostate cancer (2). In several independent studies, TECPR1 was shown to play a role in autophagy through interaction with Atg5 (3-5). Atg5 is a protein that is conjugated to the ubiquitin-like protein Atg12 and plays an essential role in autophagy (6). Initial studies suggested that TECPR1 plays a role in selective autophagy processes by targeting bacterial pathogens, as well as damaged mitochondria and protein aggregates (4). TECPR1 appears to be important for autophagosome maturation and promotes autophagosome fusion with the lysosome (5). Deletion of TECPR1 leads to an accumulation of autophagosomes (5).

1. Jeynov, B. et al. (2006) FEBS Lett 580, 5917-24.
2. Bull, J.H. et al. (2001) Br J Cancer 84, 1512-9.
3. Behrends, C. et al. (2010) Nature 466, 68-76.
4. Ogawa, M. et al. (2011) Cell Host Microbe 9, 376-89.
5. Chen, D. et al. (2012) Mol Cell 45, 629-41.
6. Mizushima, N. et al. (1998) Nature 395, 395-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody