Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)

• 产品编号：CST-8095S      品牌：CST       原厂货号：8095S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)包含高亲和性的珠子。ELISA中对大量磷酸化肽段的试验显示P-Tyr-100结合磷酸化酪氨酸，无论周围的氨基酸序列。二维凝胶Western Blot分析过钒酸盐处理处理的细胞抽提物也显示P-Tyr-100与大量酪氨酸磷酸化的蛋白质发生相互作用。P-Tyr-100不与其它包含磷酸化丝氨酸和磷酸化苏氨酸的肽段发生相互作用。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）该单克隆抗体用包含磷酸化酪氨酸的肽段免疫动物制备。CST生产的此抗体通过共价结合肼基烟酰胺修饰的抗体与甲酰苯甲酰胺修饰的磁珠而固定。Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)用于免疫共沉淀分析。未结合的Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411可以与所有种属的磷酸化酪氨酸蛋白结合。CST预计Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)也可以识别所有物种的磷酸化蛋白。酪氨酸磷酸化在细胞信号转导中扮演重要角色（1）。在肿瘤中，上调酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成（2）。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值（3,4）。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体是非常灵敏的新工具，在高通量药物发现中检测酪氨酸磷酸化和酪氨酸激酶活性研究中的使用逐渐增加。

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

原厂资料：

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) contains high affinity beads. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.

Description

This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) is useful for immunoprecipitation assays. The unconjugated Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 reacts with all species of phospho-tyrosine protein. CST expects that Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) will also recognize phospho proteins in all species.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

注意事项：

Storage: Supplied in PBS Buffer (pH 7.2), 0.1% Tween-20.
Store at 4ºC. Do not aliquot the antibody.